摘要
目的探讨尾静脉、腹主动脉和下腔静脉取血对成年Wistar大鼠部分血液生物化学指标的影响。方法 34只成年Wistar大鼠按性别等分2组,各组麻醉后依次采集尾静脉,腹主动脉和下腔静脉血,血清用7180E型全自动生物化学分析仪测定11种血液生物化学指标,包括丙氨酸氨基转移酶(alanine aminotransferase,ALT)、门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、尿素氮(urea nitrogen,BUN)、血糖(glucose,GLU)、总蛋白(total protein,TP)、白蛋白(albumin,ALB)、血肌酐(creatinine,Cr)、三酰甘油(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoproteincholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)。结果在雌性Wistar大鼠中,尾静脉和下腔静脉血中GLU,TP和Cr差异有统计学意义(P<0.05);尾静脉和腹主动脉血中GLU、TP、ALB、Cr、TG、TC和HDL-C差异有统计学意义(P<0.05)。雄性Wistar大鼠中,尾静脉和下腔静脉血中ALT、AST、GLU、TP和Cr差异有统计学意义(P<0.05);尾静脉和腹主动脉血中ALT、AST、GLU、TP、ALB、TG、TC、HDL-C和LDL-C差异有统计学意义(P<0.05)。不同性别之间:尾静脉取血时AST、TC和LDL-C差异有统计学意义(P<0.05);下腔静脉取血时TP、ALB、Cr、TG、TC和LDL-C差异有统计学意义(P<0.05);腹主动脉取血时TP、ALB、Cr、TC和LDL-C差异有统计学意义(P<0.05)。结论不同取血方式和性别会影响成年Wistar大鼠的血液生物化学指标。
Objective To establish the influence of different sampling methods and gender on 11 biochemical indicators in Wistar rat.Methods The blood of adult Wistar rats （17 males and 17 females） were collected from caudal vein, abdominal aorta and inferior vena cava, then 11 biochemical indicators including alanine aminotransferase （ALT）, aspartate aminotransferase（AST）, urea nitrogen （BUN）, glucose （GLU）, total protein （TP）, albumin（ALB）, creatinine （Cr）, triglyceride （TG）, total cholesterol （TC）, high-density lipoprotein cholesterol （HDL-C） and low-density lipoprotein cholesterol （LDL-C） were analyzed by 7180E Automatic Biochemical Analyzer.Means comparison were performed byt-test or Mann-Whitney U-test depending on the distribution of the data.A value 〈 0.05 was regarded as statistical significance.Results In female rats, significant variation was observed on the value of GLU, TP and Cr between caudal vein and inferior vena cava serums;and significant difference was observed on the values of GLU, TP, ALB, Cr, TG, TC and HDL-C between caudal vein and abdominal aorta serums.In male rats, the value of ALT, AST, GLU, TP and Cr were significantly different between caudal vein and inferior vena cava serums;and significant difference was observed on the values of ALT, AST, GLU, TP, ALB, TG, TC, HDL-C and LDL-C between caudal vein and abdominal aorta serums.Between male and female rats, the value of AST, TC and LDL-C were significantly different in caudal vein serum;and significant variation on the value of TP, ALB, Cr, TG, TC and LDL-C were found;and the value of TP, ALB, Cr, TC and LDL-C were statistically different.Conclusion Researchers should pay more attention to the variation of biochemical indicators caused by the different sampling methods and gender in Wistar rat.
出处
《首都医科大学学报》
CAS
北大核心
2017年第4期572-575,共4页
Journal of Capital Medical University
基金
首都医科大学校长基金技术类(2015JS29)~~
