摘要
目的:建立高效液相色谱法(HPLC)同时测定不同产地乌药叶中4种倍半萜内酯(hydroxylinderstrenolide,6-acetyl lindenanolide B1 and B2,atractylenolide Ⅲ,lindenanolide H)的含量。方法:采用Inertsustain C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸水溶液为流动相,梯度洗脱(0~5 min,10%A→30%A;5~40 min,30%A→90%A;40~45 min,90%→10%A;45~50 min,10%A),流速为1.0 m L/min,检测波长210 nm,柱温30℃,进样量10μL。结果:4种倍半萜内酯在测定质量浓度范围内与峰面积呈良好的线性关系,相关系数均在0.9998以上,有良好的精密度、稳定性、重复性和回收率;不同产地乌药叶中倍半萜内酯含量差异显著,4种倍半萜内酯质量分数的总和在0.71~2.43 mg/g之间。结论:该方法简单、准确、重复性好,对于乌药叶的质量评价、质量标准的建立均具有一定参考意义。
Objective To establish an HPLC method for simultaneous determination of four kinds of sesquiterpene lactones including the hydroxylinderstrenolide,6-acetyl lindenanolide B1 and B2,atractylenolide III and lindenanolide H in the leaves of Lindera aggregata(Sims)Kosterm.from different origins.Methods The HPLC separation was performed on an Inertsustain C 18(250 mm×4.6 mm,5μm)column.Acetonitrile(A)-0.1%phosphoric acid in water(B)was used as gradient mobile phase(0~5 min,10%A→30%A;5-40 min,30%A→90%A;40-45 min,90%→10%A;45-50 min,10%A).The flow rate was 1.0 mL/min.The column temperature was 30℃,and the detected wavelength was 210 nm.Results The linear relation of four sesquiterpene lactones measured in the range of mass concentration was good with perfect precision,repeatability,and stability,the value of which was all more than 0.9998.The contents of sesquiterpene lactones in the leaves of Lindera aggregata(Sims)Kosterm.from different origins were significantly different,and the total content of four kinds of sesquiterpene lactones was 0.71~2.43 mg/g.Conclusion The method is simple,accurate and reproducible,and has certain significance for the quality evaluation and control of the leaves of Lindera aggregata(Sims)Kosterm.
作者
孙崇鲁
章璐幸
俞松林
李士敏
SUN Chonglu;ZHANG Luxing;YU Songlin;LI Shimin(Zhejiang Pharmaceutical College,Ningbo 315100,China)
出处
《中国民族民间医药》
2022年第12期58-62,共5页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
宁波市自然基金项目(2019A610374)。
关键词
HPLC
乌药叶
倍半萜内酯
定量分析
HPLC
Linderae Aggregate Leaf
Sesquiterpene Lactones
Quantitative Analysis
作者简介
孙崇鲁(1981-),男,汉族,硕士,高级实验师,研究方向为天然产物分离纯化。E-mail:scl267@163.com。
