摘要
【目的】明确大蛾卵跳小蜂雌雄成虫触角感受器的类型、数量和分布特征,为探究各感受器的内部结构、感受机制及相关功能提供基础。【方法】利用扫描电镜观察大蛾卵跳小蜂的触角结构、长度及触角感受器的形态特征;鉴定感受器的类型,统计触角背面和腹面不同类型感受器的数量、位置。通过独立样本t检验比较触角长度和同类感受器的长度、宽度、数量在雌、雄蜂间的差异;比较不同亚节上感受器的数量、分布密度在雌、雄蜂间的差异。【结果】大蛾卵跳小蜂触角呈膝状,雌蜂的触角总长[(806.79±4.30)μm]大于雄蜂触角总长[(781.13±7.13)μm]。雌蜂触角鞭节有9亚节,包括6索节和3棒节;雄蜂触角鞭节有7亚节,包括6索节和1棒节。雌、雄蜂触角上都着生7种类型感受器,分别为毛形感受器(TS)、刺形感受器(ChS)、钟形感受器(CaS)、板形感受器(PS)、乳状感受器(MS)、B?hm氏鬃毛(BB)、指形感受器(FS)。TS和FS都分为Ⅰ型和Ⅱ型。雌蜂和雄蜂上TS的数量最多、分布最广,TSⅠ和TSⅡ在雌雄间的数量无显著差异。雌蜂ChS、FSⅠ的数量显著大于雄蜂相同类型的感器数量;雌蜂BB、FSⅡ的数量显著小于雄蜂相同类型的感器数量。PS、CaS、MS的数量在雌雄蜂间无显著差异。观察发现,从触角基部到端部,感受器类型和数量越来越多,密度越来越大。【结论】雌、雄蜂触角感受器的总量较为接近,但不同感受器数量、大小和分布存在差异,具有明显的性二型现象;跳小蜂科昆虫中存在钟形感受器;大蛾卵跳小蜂的触角鞭节可能在寄主定位、识别、产卵等过程中起到更重要的作用。
【Objective】 The type, number and distribution characteristics of the antennal sensilla of male and femaleOoencyrtus kuvanaewere determined, to provide a basis for exploring the internal structure, sensing mechanisms and related functions of each type of sensilla.【Method】 The antenna structure, length and morphological characteristics of antennal sensilla ofO. kuvanaewere observed using scanning electron microscopy(SEM). The types of sensilla were identified, the number and location were counted for different types of sensilla on the back and ventral surfaces of the antennae. The length of the antenna and the length, width and number of the same type of sensilla between the female and male were compared using an independent samplettest. The differences in the number and distribution density of the sensilla on different antennal segments were compared between the female and male.【Result】 The antennae ofO.kuvanaewere geniculate and the antennae length of the female(806.79 ± 4.30) μm was larger than that of the male (781.13 ± 7.13) μm. The flagellum of the female had 9 segments, including 6 funicles and 3 clavae. The flagellum ofthe male comprised 7 segments, including 6 funicles and 1 clava. There were 7 types of sensilla on the antennae of boththe female and male, including trichomes sensilla( TS), chaetica sensilla( ChS), campullacea sensilla( CaS),placodea sensilla(PS), mammilliformia sensilla(MS), B?hm bristles(BB) and finger-like sensilla(FS). The TS andFS were divided into typeⅠand typeⅡ. The numbers of TS on the female and male were the largest and the most widelydistributed. There was no significant difference in the numbers of TSⅠand TSⅡbetween the female and male. Thenumbers of ChS and FSⅠon the female were significantly greater than that on the male. The numbers of BB and FSⅡonthe female were significantly less than that on the male. There was no significant difference in the numbers of PS, CaSand MS between the female and male. The types, number and density of sensilla on the antennae increased from the baseto the end.【Conclusion】The total quantity of antennal sensilla on the female and male were similar, but the number,size and distribution of each type of sensilla were different, with pronounced sexual dimorphism. The CaS was found inEncyrtidae. The flagellum ofO. kuvanaemay play a more important role in the process of host localization, recognitionand oviposition.
作者
王建军
王敬贤
栾庆书
都慧
姜旭
董莉莉
曹传旺
WANG Jianjun;WANG Jingxian;LUAN Qingshu;DU Hui;JIANG Xu;DONG Lili;CAO Chuanwang(College of Forestry,Northeast Forestry University,Harbin 150040,China;Liaoning Provincial Key Laboratory of Forest Protection,Liaoning Academy of Forestry Sciences,Shenyang 110032,China;Anshan City Natural Resources Services Centre of Liaoning Province,Anshan 114000,China)
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第3期157-164,共8页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
沈阳市中青年科技创新人才项目(RC190103)
辽宁省农业科学院院长基金项目(2020QN2420)
辽宁省自然科学基金面上项目(2021-MS-053)。
关键词
大蛾卵跳小蜂
寄生蜂
舞毒蛾
触角感受器
扫描电镜
性二型
Ooencyrtus kuvanae
parasitoid
Lymantria dispar
antennal sensilla
scanning electron microscopy(SEM)
sexual dimorphism
作者简介
第一作者:王建军(jjw653723@126.com),高级工程师;通信作者:王敬贤(wangjingxian_1985@163.com),教授级高级工程师。
