摘要
目的在大型国际性展会期间,监测会场的生物气溶胶浓度变化,并实时检测生物气溶胶中微生物核酸。方法2021年8月30日-9月2日和2021年9月10-13日,利用自主研发的生物有害因子全自动采集与监测一体化系统,在会场内3个监测点实时监测1~5μm的生物粒子浓度,同时自动检测动物、植物、细菌、真菌管家基因和新冠病毒核酸。结果在相对稳定的环境下,会场生物气溶胶浓度低于10 ppl,在人员流动频繁时增加到最高6206 ppl。一体化系统共启动61次自动核酸检测,其中管家基因检出率分别为细菌27.9%(17/61)、植物13.1%(8/61)、真菌6.6%(4/61)、动物0%。检测到3次(4.9%)新冠病毒核酸,经复核均为假阳性。结论会场环境空气质量较好,生物气溶胶浓度与人类活动频率正相关。细菌性气溶胶是会场内生物气溶胶的主要成分。
Objective To monitor the dynamic change of bioaerosol concentration during international exhibitions,and detect nucleic acid of microorganisms in bioaerosols.Methods The self-developed automatic pathogen detection system was used to monitor the concentration of biological particles with diameter of 1-5μm in real time,and automatically detect housekeeping genes of animals,plants,bacteria,fungi and SARS-CoV-2 at three monitoring sites from August 30 to September 2,September 10 to 13,2021.Results In a relatively stable environment,the concentration of bioaerosols was less than 10 ppl,and increased to 6206 ppl when attendants moved frequently.A total of 61 automated nucleic acid tests were performed.The positive rate of housekeeping genes was 27.9%of bacteria(17/61),13.1%of plants(8/61),6.6%of fungi(4/61)and 0%of animals(0/61).SARS-CoV-2 were detected3 times,but all were confirmed to be false positives by re-detection at laboratory.Conclusion The air quality of the exhibition hall was acceptable.The bioaerosol concentration was positively correlated with the frequency of human activities,and bacterial aerosols were main component of bioaerosols.
作者
赵可心
王盼
刘莹莹
李凡双
谢泰来
牛羽
李慧鹏
刘清扬
江笛
宋大伟
马雪征
胡孔新
ZHAO Ke-xin;WANG Pan;LIU Ying-ying;LI Fan-shuang;XIE Tai-lai;NIU Yu;LI Hui-peng;LIU Qing-yang;JIANG Di;SONG Da-wei;MA Xue-zheng;HU Kong-xin(Institute of Health Inspection and Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100176,China;不详)
出处
《中国国境卫生检疫杂志》
CAS
2022年第2期104-108,共5页
Chinese Journal of Frontier Health and Quarantine
基金
中国检验检疫科学研究院基本科研业务费项目(2020JK024)
国家重点研发计划项目(2021YFC2600503)
国家市场监督管理总局“科技助力经济2020”重点专项(2020-030)。
关键词
生物气溶胶
新冠病毒
一体机
管家基因
Bioaerosol
SARS-CoV-2
Integrative equipment
Housekeeping genes
作者简介
通信作者:胡孔新,E-mail:hukongxin@caiq.org.cn。
