摘要
【目的】研究优选大孔树脂分离纯化野山杏总黄酮的最佳工艺参数。【方法】采用紫外分光光度法测定野山杏总黄酮含量,利用静态吸附解吸试验分析4种不同型号大孔树脂对野山杏总黄酮的吸附及解吸能力,选出适宜的大孔树脂及其最佳分离纯化条件。【结果】D101型大孔树脂对野山杏总黄酮有较好的比吸附量和解析附能力,最佳分离纯化工艺条件为D-101大孔树脂径高比1∶6,野山杏粗提液最大上样量为6.3 BV,最佳上样浓度为0.3 mg/mL,上样速率为1.0 mL/min,静置时间为2 h,水洗体积为5 BV,水洗液洗脱流速1.5 mL/min,洗脱溶剂用80%乙醇,洗脱溶剂用量2 BV,洗脱剂流速为0.5 mL/min。【结论】D101型大孔树脂在所得工艺条件下可以对野山杏中总黄酮进行较好的分离纯化,可用于野山杏总黄酮的分离纯化。
【Objective】To optimize the optimal process parameters for separation and purification of total flavonoids from Armeniaca sibirica(L.)Lam with macroporous resin.【Methods】Ultraviolet spectrophotometry was used to determine the total flavonoids content of Armeniaca sibirica(L.)Lam,and the static adsorption and desorption test was used to investigate the adsorption and desorption capacity of 4 different types of macroporous resins on the total flavonoids of Armeniaca sibirica(L.)Lam.The appropriate macroporous resin was selected and finally the best separation and purification conditions were determined.【Results】D101 type macroporous resin had a better specific adsorption capacity and desorption capacity for total flavonoids from Armeniaca sibirica(L.)Lam.The best separation and purification process conditions were D-101 macroporous resin with a diameter-to-height ratio of 1∶6,and the loading volume of the crude extract of Armeniaca sibirica(L.)Lam was the largest:6.3 BV,whose best loading concentration was 0.3 mg/mL,The loading rate was 1.0 mL/min,the standing time was 2 h,the washing volume was 5 BV,and the washing liquid elution flow rate was 1.5 mL/min.80%ethanol was used as the elution solvent,whose amount was 2 BV,and the eluent flow rate 0.5 mL/min.【Conclusion】D101 type macroporous resin can be used for the separation and purification of total flavonoids in Armeniaca sibirica(L.)Lam under the obtained technological conditions.
作者
金科旭
戴小华
谷虹霏
束佳敏
王天琪
海婷玉
JIN Kexu;DAI Xiaohua;GU Hongfei;SHU Jiamin;WANG Tianqi;HAI Tingyu(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2022年第4期900-907,共8页
Xinjiang Agricultural Sciences
基金
国家自然科学基金“野山杏总黄酮对LPS诱导鸡炎症的影响及其机制研究”(31960718)。
关键词
野山杏
总黄酮
D-101大孔树脂
分离纯化
吸附
Armeniaca sibirica(L.)Lam
total flavonoids
D-101 macroporous resin
separation and purification
adsorption
作者简介
金科旭(1995-),男,河南鹿邑人,硕士研究生,研究方向为兽医药理,(E-mail)2212325408@qq.com;通信作者:戴小华(1976-),女,广西陆川人,副教授,博士,硕士生导师,研究方向为天然产物功能,(E-mail)254799258@qq.com。
