摘要
目的探讨电针次髎穴对压力性尿失禁大鼠是否具有穴位特异性。方法8周龄雌性SD大鼠50只,体重180~220 g,按随机数字表法分为正常组、模型组、次髎穴组、三阴交组、非经穴组,每组10只。正常组仅检测,其余四组大鼠均先进行双侧卵巢摘除手术,继予阴道扩张手术建立压力性尿失禁模型。造模成功后,于电针干预前后行尿流动力学检测,观察大鼠腹压漏尿点压(ALPP)、最大膀胱容量(MBC)的改变。免疫组化法检测耻尾肌与膀胱颈尿道括约肌组织中Ⅰ型胶原蛋白(collagen Ⅰ)、结缔组织生长因子(CTGF)表达水平。结果尿流动力学结果显示,干预后,与正常组比较,模型组MBC、ALPP值明显下降(P<0.05);与模型组比较,干预后次髎组、三阴交组、非经穴组MBC、ALPP值升高(P<0.05);与次髎组比较,三阴交、非经穴组MBC、ALPP值降低(P<0.05)。免疫组化结果显示,与正常组比较,模型组大鼠盆底结缔组织collagen Ⅰ、CTGF的表达水平明显降低(P<0.01);与模型组比较,次髎组、三阴交组、非经穴组collagen Ⅰ、CTGF的表达水平明显升高(P<0.05),与次髎组比较,三阴交组、非经穴组collagen Ⅰ、CTGF的表达水平降低(P<0.05)。结论电针次髎穴可有效改善压力性尿失禁症状及修复其盆底组织损伤,该膀胱调节效应具有穴位特异性。
Objective To investigate the acupuncture point specificity of electroacupuncture at the cìliáo point in rats with stress incontinence.Methods Fifty 8-week-old female SD rats,weighing 180-220 g,were divided into normal group,model group,cìliáo point group,sānyīnjiāo group,and non-channel point group according to random number table method,with ten rats in each group.The normal group was only tested,and the other four groups were treated with bilateral ovariectomy first,followed by vaginal dilation to establish the model of stress incontinence.After modeling,urine flow dynamics was detected before and after electroacupuncture intervention,and the changes of abdominal leak point pressure(ALPP)and maximal bladder capacity(MBC)were observed.Immunohistochemistry was used to detect the expression levels of type Ⅰ collagen(collagen Ⅰ)and connective tissue growth factor(CTGF)in the tissues of caudal gluteus muscle and bladder cervical urethral sphincter.Results The results of urine flow dynamics showed that MBC and ALPP values of model group were significantly decreased after intervention compared with normal group(P<0.05).Compared with model group,MBC and ALPP values of cìliáo point group,sānyīnjiāo group,and non-channel point group were increased after intervention(P<0.05).Compared with cìliáo point group,MBC and ALPP values of sānyīnjiāo group and non-channel point group were decreased(P<0.05).Immunohistochemical results showed that compared with normal group,the expression levels of collagen Ⅰ and CTGF in model group were significantly decreased(P<0.01).Compared with model group,the expression levels of collagen Ⅰ and CTGF in cìliáo point group,sānyīnjiāo group,and non-channel point group were significantly increased(P<0.05).Compared with the cìliáo point group,the expression levels of collagenⅠand CTGF in sānyīnjiāo group and non-channel point group were decreased(P<0.05).Conclusion Electroacupuncture on cìliáo point can effectively improve the symptoms of stress urinary incontinence and repair the pelvic floor tissue damage,and the bladder regulation effect has acupoint specificity.
作者
张薇薇
蔡明星
莫倩
杨丹
ZHANG Weiwei;CAI Mingxing;MO Qian;YANG Dan(College of Acupuncture and Massage,Guizhou University of Traditional Chinese Medicine,Guizhou Province,Guiyang 550002,China;Department of Acupuncture,the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guizhou Province,Guiyang 550001,China)
出处
《中国医药导报》
CAS
2022年第10期5-8,13,共5页
China Medical Herald
基金
国家自然科学基金资助项目(81760895、81660822)。
关键词
压力性尿失禁
电针
次髎
穴位特异性
Stress incontinence
Electroacupuncture
cìliáo
Acupuncture point specificity
作者简介
张薇薇(1995.10-),女,贵州中医药大学针灸推拿学院2019级针灸推拿学专业在读硕士研究生,研究方向:针灸治疗下尿路疾病;通讯作者:杨丹(1966.12-),女,教授,研究方向:针灸治疗心脑血管系统疾病。
