摘要
建立了UHPLC-QTOF/MS^(E)法在正、负离子2种模式下检测苓桂术甘汤中的主要化学成分,并结合自建数据库和在线数据库,通过准确相对分子质量、碎片离子信息、裂解规律和对照品比对等方式鉴定苓桂术甘汤中化学成分。同时,应用UNIFI^(TM)的二元比较功能实现了各化合物的归属。结果显示,共从苓桂术甘汤中鉴定出100个化合物,包括6个氨基酸类、4个糖类、11个小分子有机酸类、27个黄酮类、40个皂苷类和12个其他类成分,较为全面地反映了苓桂术甘汤中具有药理活性的化学物质和各个药材的特征性成分,为苓桂术甘汤中化学物质的快速鉴定、质量控制与临床合理应用提供了数据参考。
An UHPLC-QTOF/MS^(E) method was established to analyze the main chemical components in Linggui Zhugan decoction in both positive and negative ion modes.Combined with the self-built databases and online databases,the chemical components in Linggui Zhugan decoction were identified by accurate relative molecular mass,fragment ion information,fragmentation law and comparison with the reference substances.In addition,the attribution of each compound was realized by using the binary comparison function of UNIFI^(TM).As a result,a total of one hundred compounds were identified from Linggui Zhugan decoction,including six amino acids,four carbohydrates,eleven small molecular organic acids,twenty-seven flavonoids,forty saponins and twelve other components.It showed that the pharmacologically active chemical substances in Linggui Zhugan decoction and the characteristic components of each medicinal material were comprehensively reflected,which provided a data reference for the rapid identification,quality control and clinical application of the chemical substances in Linggui Zhugan decoction.
作者
许雅婧
吴彤
张志强
林珊
XU Yajing;WU Tong;ZHANG Zhiqiang;LIN Shan(State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 201203;Beijing Kangrentang Pharmaceutical Co.,Ltd.,Beijing 101301)
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2022年第2期253-263,共11页
Chinese Journal of Pharmaceuticals
作者简介
许雅婧(1996-),女,硕士研究生,专业方向:中药分析;通信作者:林珊(1990-),女,助理研究员,从事中药物质基础与质量标准的研究与开发。E-mail:18019100383@163.com。
