摘要
目的 研究 II 期宫颈鳞癌患者亚甲基四氢叶酸还原酶 (methylenetetrahydrofolate reductase,MTHFR) 和谷胱甘肽 S 转移酶 P1(glutathione S transferase P1, GSTP1) 基因多态性与化疗敏感度及预后的相关性。方法 选取 2015 年1 月~ 2016 年 1 月西北妇女儿童医院收治的 80 例行顺铂联合 5 氟尿嘧啶(PF)化疗方案的 II 期宫颈鳞癌患者为研究对象,检测 MTHFR -rs1801131 和 GSTP1- rs1695 位点的多态性,并分析其与患者化疗敏感度及 5 年生存率的相关性。结果 II 期宫颈鳞癌患者的 MTHFR- rs1801131 和 GSTP1- rs1695 位点的分布符合 Hardy-Weinberg 平衡 (χ^(2)=2.069,5.123,均 P > 0.05),具有群体代表性。MTHF- rs1801131 位点中 AA 基因型(77.50%)占比高于 AC(12.50%)和 CC(10.00%)基因型 (χ^(2)=105.450,P < 0.001),GSTP1- rs1695 位点中 AA 基因型(65.00%)占比高于 AG(23.75%)和 GG(11.25%)基因型 (χ^(2)=56.963,P < 0.001),差异均有统计学意义。MTHFR -rs1801131 位点 AA 基因型化疗敏感度为 24.19%,AC/CC 型为 33.33%,其差异无统计学意义 (χ^(2)=0.602,P=0.438)。GSTP1- rs1695 位点 AA 基因型和 AG/GG 基因型的化疗敏感度分别为 17.31% 和 39.29%,G 等位基因型对化疗的敏感度更强,且差异具有统计学意义 (χ^(2)=4.689,P=0.030)。此外,MTHFR- rs1801131 位点 AA 基因型患者 5 年生存率为 75.81%,AC/CC 型为 50.00%。与 AA 基因型相比,AC/CC 型患者的 5 年生存率较短 (Log-rank=5.035,P=0.024 8)。GSTP1- rs1695 位点 AA 基因型和 AG/GG 基因型的 5 年生存率分别为 78.85% 和 71.43%,差异无统计学意义 (Log-rank=0.594 0,P=0.440 9)。结论 MTHFR- rs1801131 位点的多态性可影响 II 期宫颈鳞癌患者的化疗预后,GSTP1- rs1695 位点的多态性则与 II 期宫颈鳞癌患者的化疗敏感度相关。
Objective To study the correlation between methylenetetrahydrofolate reductase (MTHFR) and glutathione S transferase P1 (GSTP1) gene polymorphisms and chemosensitivity and prognosis in patients with stage II cervical squamous cell carcinoma. Methods 80 patients with stage II cervical squamous cell carcinoma treated with PF chemotherapy regimen in Northwest Women’s and Children’s Hospital from January 2015 to January 2016 were selected as the research object. The polymorphisms of MTHFR-rs1801131 and GSTP1-rs1695 loci were detected, and their correlation with chemotherapy sensitivity and 5-year survival rate were analyzed. Results The distribution of MTHFR-rs1801131 and GSTP1-rs1695 in stage II cervical squamous cell carcinoma patients accorded with Hardy Weinberg equilibrium (χ^(2)=2.069,5.123, all P > 0.05).The proportion of AA(77.50%)genotype in MTHFR-rs1801131 was higher than that in AC(12.50%)and CC (10.00%)genotypes,(χ^(2)=105.450,P < 0.001), and the proportion of AA (65.00%)genotype in GSTP1-rs1695 locus was higher than that in AG(23.75%)and GG(11.25%) genotypes (χ^(2)=56.963, P < 0.001),the differences were statistically significant,respectively.According to the definition of chemosensitivity, it was found that the chemosensitivity of AA genotype at MTHFR-rs1801131 was 24.19%, and that of AC/CC genotype was 33.33%, the difference was not statistically significant (χ^(2)=0.602, P=0.438). The chemosensitivity of AA genotype and AG/GG genotype at GSTP1-rs1695 locus were 17.31% and 39.29%,respectively. G allele was more sensitive to chemotherapy, and the difference was statistically significant (χ^(2)=4.689, P=0.030). The 5-year survival rate of AA genotype at MTHFR-rs1801131 locus was 75.81% and that of AC/CC genotype was 50.00%. Compared with AA genotype, the 5-year survival rate of patients with AC/CC genotype was shorter (log rank=5.035, P=0.0248). The 5-year survival rates of AA genotype and Ag / GG genotype at GSTP1-rs1695 locus were 78.85% and 71.43%, respectively. There was no significant difference (log rank=0.594 0, P=0.440 9). Conclusion the polymorphism of MTHFR-rs1801131 locus could affect the chemotherapy prognosis of patients with stage II cervical squamous cell carcinoma, and the polymorphism of GSTP1-rs1695 locus was related to the chemotherapy sensitivity of patients with stage II cervical squamous cell carcinoma.
作者
朱家凤
苏琦
ZHU Jia-feng;SU Qi(Department of Pharmacy,Northwest Women’s and Children's Hospital,Xi’an 710061,China)
出处
《现代检验医学杂志》
CAS
2022年第2期54-57,60,共5页
Journal of Modern Laboratory Medicine
关键词
宫颈鳞癌
亚甲基四氢叶酸还原酶
谷胱甘肽S转移酶P1
cervical squamous carcinoma
methylenetetrahydrofolate reductase
glutathione S transferase P1
作者简介
朱家凤(1989-),女,本科,主管药师,研究方向:临床药学,E-mail:562600345@qq.com;通讯作者:苏琦(1986-),男,本科,主管药师,研究方向:临床药学,E-mail:450216864@qq.com.
