摘要
[目的]研究将生长/分化因子-10(GDF10)作为候选基因,探讨其多态与不同性别的德州驴生长性状之间的关系。[方法]通过对440头德州驴进行多态检测,鉴定得到3个SNPs位点,并对SNP位点进行分型。利用SPSS 18.0软件分析3个SNPs位点的不同基因型与德州驴9个生长性状的关联分析。[结果]结果显示,3个位点均处于Hardy-Weinberg平衡状态(P>0.05);群体遗传参数分析发现,GDF10基因的SNP位点的纯合度较高,多态信息含量均处于中度多态(0.25G位点AA基因型个体的尻高显著高于GA基因型个体(P<0.05);g.2041898A>G位点AA基因型个体的体高和尻长显著高于GA基因型个体(P<0.05);g.2043035 C>T位点上CC基因型个体的尻长显著高于CT基因型个体(P<0.05)。在公驴中,g.2043035 C>T位点CC基因型个体的体高显著低于CT基因型(P<0.05)。[结论]综上所述,GDF10基因多态性与德州驴的部分生长性状相关,有潜力作为德州驴的生长发育性状分子标记。
[Objective] In this study, growth/differentiation factor-10(GDF10) was selected as a candidate gene to explore the association between GDF10 polymorphism and growth traits of Dezhou donkeys of different genders. [Method] In 440 Dezhou donkeys, 3 SNPs were detected, and were genotyped. SPSS 18.0 software was used to analyze the association between three genotypes at different SNP loci and nine growth traits of Dezhou donkeys. [Result] The results showed that the three loci were in hardy-Weinberg equilibrium(P> 0.05). Analysis of population genetic parameters showed that there is a high degree of homogeneity in the polymorphic loci of GDF10 gene, and the content of polymorphic information was moderate(0.25< PIC< 0.50). The results of association analysis showed that in jennies, the rump high of AA genotype was significantly higher than that of GA genotype at g.2031481 A>G locus(P<0.05), at g.2041898 A>G locus, the body height and rump length of AA genotype were significantly higher than those of GA genotype(P<0.05), and at g.2043035 C>T locus, the rump length of CC genotype was significantly higher than that of CT genotype(P<0.05). In jackasses, the body height of CC genotype at the g.2043035 C>T locus was significantly lower than that of CT genotype(P<0.05). [Conclusion] In conclusion, GDF10 gene polymorphism is related to the growth traits of Dezhou donkeys and can be used as a molecular marker for the growth and development of Dezhou donkeys.
作者
白福霞
李梅
裴浩宇
安小娅
王刚
王延涛
于杰
党瑞华
BAI Fu-xia;LI Mei;PEI Hao-yu;AN Xiao-ya;WANG Gang;WANG Yan-tao;YU Jie;DANG Rui-hua(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100;Dong-E-E-Jiao Co.Ltd.,No.78,E-jiao Street,Dong-E Country,Shandong 252201)
出处
《中国牛业科学》
2022年第1期65-72,共8页
China Cattle Science
基金
陕西省重点研发计划项目(2020NY-002)
延安市科技计划项目(2019ZDQY-018,2019ZDQY-005,2019ZDQY-016)
东阿阿胶公司项目(20191001)
国家自然科学基金面上项目(81770514)。
作者简介
白福霞(1997-),女,硕士研究生,主要从事动物遗传育种研究;通讯作者:党瑞华(1976-),男,博士,副教授,主要从事畜禽经济性状遗传基础及疾病易感基因研究。
