摘要
[目的]克隆金川牦牛低氧诱导因子-3A(HIF-3A)并进行生物信息学的分析。[方法]采用PCR方法从金川牦牛血液中克隆出HIF-3A,通过生物学软件Protparam、SOPMA、ProtScale、NetPhos、SignaIP、TMHMM、PSORTⅡ、Protfun2.2对核酸序列以及蛋白进行分析。[结果]金川牦牛HIF-3A长度为513 bp、ORF为441 bp、一共编码氨基酸146个,相对分子质量为15 729.19 Da,和普通牛的CDS区有8个碱基不同,突变了5个氨基酸,预测无跨膜区域,不存在信号肽序列,主要位于细胞质与线粒体中,有β-转角和α-螺旋。在同源性比较中与牛的同源性达到了97.5%,与其他物种同源性较低。[结论]成功克隆出金川牦牛的HIF-3A基因,且不同属的物种间不具有较高的保守性,为非分泌蛋白。
[Objective]To clone the hypoxia-inducible factor-3 a（ HIF-3 A） in Jinchuan yak and analyze the bioinformatics. [Methods] HIF-3 A was cloned from the blood of Jinchuan yak by PCR. The nucleic acid sequences and proteins were analyzed by bioproducts Protparam,SOPMA,Prot Scale,NetPhos,SignaIP,TMHMM,PSORT Ⅱ,Protfun 2. 2. [Results] The length of HIF-3 A was 513 bp and the ORF was 441 bp. The total molecular weight was 15 729. 19 Da,which was different from that of the CDS region of the common cattle. The mutation was 5 amino acids,Predicted no transmembrane region,there is no signal peptide sequence,mainly located in the cytoplasm and mitochondria,with β-turn and α-helix. In the homology comparison with the cattle homology reached 97. 5%,with other species of low homology. [Conclusion] The HIF-3 A gene of Jinchuan yak was successfully cloned,and the non-secreted protein was not conserved among different species.
出处
《生物技术》
北大核心
2017年第5期472-477,共6页
Biotechnology
基金
西南民族大学研究生创新性科研项目(No.CX2017SZ029)
四川省教育厅重点项目("金川牦牛低氧适应的分子遗传机制研究"
No.16ZA0014)
关键词
金川牦牛
低氧诱导因子-3a
克隆
生物信息学分析
Jinchuan yak, hypoxia - inducible factor - 3 a, cloning, bioinformatics analysis
作者简介
李晨阳（1993-），男，山西侯马人，硕士生，研究方向：动物遗传学，Email：2273522704@qq．com。
通讯作者：王利（1977-），女，四川成都人，博士，副教授，研究方向：动物遗传学，发表论文60余篇，Email：qinxin916@aliyun．com。
