摘要
Xiaoer-Feire-Kechuan(XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation.The complicated composition rendered its chemical analysis and effective-component elucidation.In this study,we combined quantitative analysis and bioactivity test to reveal the antiinflammatory constituents of XFK.First,UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes(covering 9 out of 11 herbs) in different XFK formulations.Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity.Then,anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay.Finally,major analytes forsythosides H,I,A(8-10),and baicalin(15)(total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities(inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK.The present study provided an effective strategy to discover effective constituents of multi-herb formulas.
基金
supported by the National Key Research and Development Program of China (Grant No.: 2018YFC1707304, 2018YFC1707301)
Beijing Natural Science Foundation (Grant No.: JQ18027)
National Natural Science Foundation of China (Grant No.: 81725023)。
作者简介
Corresponding author:Min Ye,E-mail addresses:yemin@bjmu.edu.cn;Corresponding author:Xue Qiao,E-mail addresses:qiaoxue@bjmu.edu.cn。
