摘要
目的研究肝癌外周血单核细胞表面TLR2、TLR4水平与小肠细菌过度生长的关系。方法研究对象为2017年3月至2019年3月在我院治疗的45例乙肝相关性肝癌患者与35例慢性乙型肝炎患者以及15例健康体检患者,分别设为研究组A、研究组B与参照组。对研究组A、研究组B与参照组的TLR2、TLR4水平与小肠细菌生长状态进行分析。结果研究组A的SIBO阳性率明显高于研究组B与参照组,研究组B的SIBO阳性率高于参照组;研究组A的外周血CD_(14)^(+)单核细胞表面TLR2^(+)、TLR4^(+)细胞所占的比例均高于研究组B与参照组(P<0.05);研究组B的细胞所占的比例与参照组差异有统计学意义(P<0.05);研究组A的外周血CD_(14)^(+)单核细胞表面TLR4^(+)细胞GMF显著高于研究组B与参照组(P<0.05),研究组B的外周血CD_(14)^(+)单核细胞表面TLR4^(+)细胞GMF参照组比较(P<0.05);研究组A的血浆内毒素水平均高于研究组B、参照组;乙肝肝癌合并SIBO阳性者内毒素水平明显高于SIBO阴性患者(P<0.05)。结论乙肝相关性肝癌的SIBO发生率较高,合并SIBO患者的血浆内毒素与TLR2、TLR4表达水平升高。在肝癌发生与发展期间,SIBO患者TLR2、TLR4水平的高表达具有十分积极的作用。
Objective To study the relationship between TLR2 and TLR4 levels on the surface of monocytes in peripheral blood of liver cancer and the overgrowth of small intestinal bacteria.Methods The researches subjects were 45 cases of HBV-related liver cancer patients treated in our hospital from March 2017 to March 2019,35 cases of chronic hepatitis B patients,and 15 cases of healthy physical examination patients,respectively divided into study group A,study group B and reference group.TLR2 and TLR4 levels in study group A,study group B and control group and the growth status of small intestinal bacteria were analyzed.Results The SIBO positive rate of study group A was significantly higher than that of study group B and the control group,and the SIBO positive rate of study group B was higher than that of the control group.The proportion of TLR2^(+)and TLR4^(+)cells on the surface of CD_(14)^(+)monocytes in study group A was higher than that in study group B and control group(P<0.05).The proportion of cells in study group B was statistically significant different from that in the control group(P<0.05).The peripheral blood CD_(14)^(+)monocyte surface TLR4^(+)cell GMF of study group A was significantly higher than that of study group B and the reference group(P<0.05),and the comparison of peripheral blood CD_(14)^(+)monocyte surface TLR4^(+)cell GMF of study group B was significant(P<0.05).The plasma endotoxin level in study group A was higher than that in study group B and control group.The endotoxin level of hepatitis B liver cancer with SIBO-positive patients was significantly higher than that of SIBO-negative patients(P<0.05).Conclusion The incidence of SIBO in HBVassociated hepatocellular carcinoma was high,and the levels of plasma endotoxin,TLR2 and TLR4 in patients with SIBO were increased.High levels of TLR2 and TLR4 in SIBO patients have a positive effect on the development and progression of liver cancer.
作者
王楠
李梦华
张锦伟
WANG Nan;LI Menghua;ZHANG Jinwei(Dalian Public Health Clinical Center(Dalian Sixth People's Hospital),Dalian 116031,China)
出处
《中国医药指南》
2022年第10期5-8,共4页
Guide of China Medicine
关键词
肝癌外周血单核细胞
TLR2
TLR4
小肠细菌
过度生长
Hepatocellular carcinoma peripheral blood monocytes
TLR2
TLR4
Small intestinal bacteria
Overgrowth
