摘要
Development of thoracolumbar vertebra(TLV)and rib primordium(RP)is a common evolutionary feature across vertebrates,although whole-organism analysis of the expression dynamics of TLV-and RP-related genes has been lacking.Here,we investigated the single-cell transcriptome landscape of thoracic vertebra(TV),lumbar vertebra(LV),and RP cells from a pig embryo at 27 days post-fertilization(dpf)and identified six cell types with distinct gene expression signatures.In-depth dissection of the gene expression dynamics and RNA velocity revealed a coupled process of osteogenesis and angiogenesis during TLV and RP development.Further analysis of cell type-specific and strand-specific expression uncovered the extremely high level of HOXA103′-UTR sequence specific to osteoblasts of LV cells,which may function as anti-HOXA10-antisense by counteracting the HOXA10-antisense effect to determine TLV transition.Thus,this work provides a valuable resource for understanding embryonic osteogenesis and angiogenesis underlying vertebrate TLV and RP development at the cell type-specific resolution,which serves as a comprehensive view on the transcriptional profile of animal embryo development.
基金
This work was supported by the Strategic Pioneer Program of the Chinese Academy of Sciences(Grant No.XDA24010107)
the Ministry of Agriculture of China(Grant No.2016ZX08009003-006)
the China Agriculture Research System(Grant No.CARS-35)
the Agricultural Science and Technology Innovation Project,China(Grant No.ASTIP-IAS02)
This work was supported by the Animal Branch of the Germplasm Bank of Wild Species,Chinese Academy of Sciences(the Large Research Infrastructure Funding).
作者简介
Corresponding authors:Yaping Zhang,E-mail:zhangyp@mail.kiz.ac.cn,ORCID:0000-0002-5401-1114;Corresponding authors:Haibing Xie,E-mail:xiehb@mail.kiz.ac.cn,ORCID:0000-0003-4977-8270;Corresponding authors:Tang Hai,E-mail:haitang@ioz.ac.cn,ORCID:0000-0002-3192-0753;Corresponding authors:Lixian Wang,E-mail:iaswlx@263.net,ORCID:0000-0001-8943-4697;Jianbo Li,Equal contribution,ORCID:0000-0002-6431-6421;Ligang Wang,Equal contribution,ORCID:0000-0002-4376-8373;Dawei Yu,Equal contribution,ORCID:0000-0002-4504-7254;Junfeng Hao,Equal contribution,ORCID:0000-0002-8209-5247;Longchao Zhang,ORCID:0000-0002-4520-3165;Adeniyi C.Adeola,ORCID:0000-0001-5171-7878;Bingyu Mao,ORCID:0000-0002-7993-3158;Yun Gao,ORCID:0000-0002-5148-7598;Shifang Wu,ORCID:0000-0001-9343-6521;Chunling Zhu,ORCID:0000-0001-7356-3419;Yongqing Zhang,ORCID:0000-0003-0581-4882;Jilong Ren,ORCID:0000-0002-2400-1951;Changgai Mu,ORCID:0000-0002-6256-9118;David M.IrwinORCID:0000-0001-6131-4933。
