摘要
随着测序技术的快速发展和测序成本的大大降低,单细胞转录组测序(Single-cell RNA-sequencing,scRNA-seq)技术已经广泛应用于猪的疾病免疫、繁殖育种、骨骼肌发育、脂肪沉积等方面。scRNA-seq是在单细胞水平上对RNA进行高通量测序和分析的新技术,能更全面、清晰地解析细胞间的异质性。本文简述了scRNA-seq技术的原理和检测分析方法,并综述了该技术在养猪生产中的应用,旨在为猪遗传基础研究提供参考。
With the rapid development of sequencing technology and the greatly reduced of sequencing costs,single-cell RNA-sequencing technology has been widely used in studies of disease immunity,reproductive breeding,skeletal muscle development and fat deposition in pig production.Single-cell RNA-sequencing is a new technology for high-throughput sequencing and analysis of RNA at the single-cell level,which can resolve the inter-cell heterogeneity more comprehensively and clearly.In this paper,we briefly introduce the principle,detection and analysis methods of this technology and review its applications in pig production with the aim of providing a reference for the basic research of pig genetics.
作者
兰运茂
曹勇杰
吴玲想
孟昕彤
邓晓
王应航
李梅
潘盈顺
赵桂英
LAN Yunmao;CAO Yongjie;WU Lingxiang;MENG Xintong;DENG Xiao;WANG Yinghang;LI Mei;PAN Yinshun;ZHAO Guiying(College of Animal Science and Technology,Yunnan Agricultural University,Yunnan Kunming 65000,China)
出处
《中国畜牧杂志》
北大核心
2025年第5期14-20,共7页
Chinese Journal of Animal Science
基金
云南省财政厅科教文化专项“云南地方猪杂交利用技术集成及产业化应用研究”(A3032022211)。
作者简介
兰运茂(2000-),男,云南昆明人,硕士研究生,主要从事猪生产、遗传育种研究,E-mail:2213973437@qq.com;通讯作者:赵桂英(1966-),女,云南昆明人,教授,主要从事猪遗传育种和地方猪种资源评价、开发及利用研究,E-mail:zhaoguiying2006@163.com。
