摘要
为研究向日葵在不同程度干旱胁迫下基因的表达调控模式,对抗旱自交系(17062)和非抗旱自交系(167072)分别进行干旱胁迫0、4、8、12、16 d以及复水处理,本研究设置干旱胁迫第8天、第16天以及复水处理与各梯度的对照组进行转录组测序分析。共设有6个差异分组,经过测序共得到277.31G的Clean data,Q30碱基>91%,平均GC含量为45.37%。样品间进行差异基因筛选,得到的DEG数量分别为2365、2571、3149、2692、4593、4980。对各差异基因进行GO和KEGG功能注释,GO分析表明,6个比较组参与生物过程差异基因较多,参与细胞组分的次之,参与分子功能的较少;KEGG分析表明干旱胁迫对向日葵的植物激素信号转导、蔗糖与淀粉代谢、氨基酸代谢有较大的影响,对生理特性的测定显示可溶性糖和可溶性蛋白与正常供水相比具有上升趋势,表明在逆境胁迫下,植物会通过对基因的表达调控的调节来改变相关生理生化代谢途径。本研究通过对向日葵叶片进行转录组分析,对揭示抗旱分子机制及相关代谢途径具有重要意义。
In order to study the gene expression regulation mode of sunflower under different degrees of drought stress,drought-resistant inbred lines(17062)and non-drought-resistant inbred lines(167072)were subjected to drought stress 0,4,8,12,16 d and rewatering respectively treatment,this study took the 8 d and 16 d of drought stress and the control group of rehydration treatment and each gradient for transcriptome sequencing analysis.There are a total of six difference groups,and a total of 277.31 G were obtained after sequencing Clean data,Q30 bases>91%,average GC content is 45.37%.Differential gene screening between samples and the number of DEGs obtained were:2365,2571,3149,2692,4593,4980.The GO and KEGG functions were annotated for each differential gene.GO analysis showed that the 6 comparison groups involved more differential genes in biological processes,followed by cell components,and less involved in molecular functions;KEGG analysis shows that drought stress has a greater impact on sunflower phytohormone signal transduction,sucrose and starch metabolism,and amino acid metabolism.The measurement of physiological characteristics shows that soluble sugar and soluble protein have an upward trend compared with normal water supply.It shows that under adversity stress,plants will change the relevant physiological and biochemical metabolic pathways through regulation of gene expression.In this study,transcriptome analysis of sunflower leaves is of great significance to reveal the molecular mechanism of drought resistance and related metabolic pathways.
作者
赵雅杰
包海柱
胡树平
赵轩微
田振东
石丰源
覃一敏
高聚林
Zhao Yajie;Bao Haizhu;Hu Shuping;Zhao Xuanwei;Tian Zhendong;Shi Fengyuan;Qin Yimin;Gao Julin(College of Agriculture,Inner Mongolia Agricultural University,Hohhot,010019)
出处
《分子植物育种》
CAS
北大核心
2022年第1期38-48,共11页
Molecular Plant Breeding
基金
内蒙古自治区自然科学基金项目(2019MS03049)
内蒙古农业大学科技成果转化专项资金动植物品种选育(培育)(YZGC2017003)
2019年内蒙古“草原英才”工程项目共同资助。
作者简介
赵雅杰,同等贡献作者;包海柱,同等贡献作者;通信作者:高聚林,nmgaojulin@163.com。
