摘要
目的优化桂枝芍药知母汤(GSZD)的提取工艺。方法采用高效液相色谱法同时测定GSZD中盐酸麻黄碱、盐酸伪麻黄碱、芒果苷、芍药苷、甘草苷、5-O-甲基维斯阿米醇苷、甘草酸、肉桂酸、6-姜酚9个成分的含量。在单因素实验的基础上,以料液比、提取次数、提取时间为考察因素,以上述9个成分含量和干膏得率作为评价指标,采用层次分析法和熵权法确定各指标的复合权重并计算综合评分,以Box-Behnken响应面法优化GSZD的提取工艺参数并验证。结果盐酸麻黄碱、盐酸伪麻黄碱、芒果苷、芍药苷、甘草苷、5-O-甲基维斯阿米醇苷、甘草酸、肉桂酸、6-姜酚含量和干膏得率的复合权重分别为0.12、0.10、0.05、0.12、0.14、0.06、0.13、0.15、0.10、0.03。GSZD的最优提取工艺为料液比1∶14(g/mL)、提取2次、提取时间3.0 h;3次验证实验的综合评分均值为95.879,RSD为0.50%(n=3),与预测综合评分(94.328)的偏差为1.64%。结论本研究确定了GSZD的最优提取工艺。
OBJECTIVE To optimize the extraction technology of Guizhi shaoyao zhimu decoction(GSZD).METHODS The contents of 9 components in GSZD were determined by HPLC,such as ephedrine hydrochloride,pseudoephedrine hydrochloride,mangiferin,paeoniflorin,liquiritin,5-O-methylvisammioside,glycyrrhizic acid,cinnamic acid,6-gingerol.On the basis of single factor experiment,taking material-liquid ratio,extraction times and extraction time as inspection factors,taking the contents of above 9 components and the yield of dry extract as evaluation indicators,the analytic hierarchy process and entropy weight method were used to determine the composite weight of each index and calculate the comprehensive score;the extraction technology parameters of GSZD were optimized by Box-Behnken response surface method,and the validation tests were conducted.RESULTS The composite weight of the contents of ephedrine hydrochloride,pseudoephedrine hydrochloride,mangiferin,paeoniflorin,glycyrrhizin,5-O-methylvisa-midol,glycyrrhizinate,cinnamic acid,6-gingerol and the yield of dry extract were respectively 0.12,0.10,0.05,0.12,0.14,0.06,0.13,0.15,0.10,0.03.The optimal extraction technology of GSZD is that the ratio of material to liquid is 1∶14(g/mL),extraction is 2 times,and the extraction time is 3.0 h;average comprehensive score of the 3 verification tests was 95.879,and RSD was 0.50%(n=3),the deviation from the predicted comprehensive score(94.328)was 1.64%.CONCLUSIONS In this study,the optimal extraction technology of GSZD is determined.
作者
郑淑晶
王佳
胡德
李阳
冷向阳
王淑敏
ZHENG Shujing;WANG Jia;HU De;LI Yang;LENG Xiangyang;WANG Shumin(School of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《中国药房》
CAS
北大核心
2022年第2期179-184,共6页
China Pharmacy
基金
吉林省科技发展计划项目(No.20190304059YY)。
关键词
桂枝芍药知母汤
提取工艺
层次分析法
熵权法
权重分析
Box-Behnken响应面法
Guizhi shaoyao zhimu decoction
extraction technology
analytic hierarchy technology
entropy weight method
weight analysis
Box-Behnken response surface method
作者简介
郑淑晶,硕士研究生。研究方向:中药健康产品研究与开发。电话:0431-86172786。E-mail:540821051@qq.com;通信作者:王淑敏,教授,博士生导师。研究方向:中药炮制、中药分析、药用微生物物质基础与药效学。电话:0431-86172786。E-mail:wangsm@ccucm.edu.cn。
