摘要
半滑舌鳎(Cynoglossus semilaevis)作为比目鱼(Pleuronectiformes)中的代表物种,是我国沿海地区重要的海水经济鱼类。半滑舌鳎体色异常的问题长期以来一直困扰着从业者。导致体色变化的原因多种多样,遗传因素是最根本原因。针对半滑舌鳎无眼侧黑化的研究多在环境、营养、生理、常见色素基因克隆等方面展开,新的功能基因挖掘工作欠缺。本研究选取半滑舌鳎不同体色皮肤进行转录组测序,通过差异基因GO和KEGG富集比对分析,在可能的6条黑色素相关的KEGG通路中筛查差异基因并对差异表达最为显著的10个基因进行了实时荧光定量聚合酶链式反应的验证。本研究找出了半滑舌鳎无眼侧黑化表达显著变化的5个功能基因,分别为txndc、alox15b、ptgs2、ptgisPTGIS、atp1a2a(p<0.05)。其中txndc、alox15b、ptgs2、ptgis基因在黑化组中表达水平高于对照组;功能方面,有3个基因均与花生四烯酸(AA)有一定的相关性,这为多不饱和脂肪酸参与半滑舌鳎体色异常的分子调控机制提供了一定的理论支持。
Cynoglossus semilaevis as a representative species of Pleuronectiformes, is an important marine economic fish in Chinese coastal areas. The abnormal body color of C. semilaevis has been perplexing the practitioners for a long time. There are many reasons leading to abnormal body color, among which the genetic factors are thought to be the most fundamental reasons. The present researches on hypermelanosis of the blind side of C. semilaevis mainly focus on environment, nutrition, physiology, cloning of known pigmental genes and so on, while the digging of new funtional genes is still lacking. In this study, skin samples with different colors of C. semilaevis are selected for transcriptome sequencing. Through GO and KEGG functional enrichment and comparative analysis of different genes, differential expressed genes are screened in six melanogenesis-related KEGG pathways and the top ten genes are verified by qPCR. In this study, we find five functional genes with significant changes in the hypermelanotic skin on the blind side of C. semilaevis, which refer to txndc, alox15 b, ptgs2, ptgis, and atp1 a2 a(p<0.05).The expression levels of txndc, alox15 b, ptgs2, and ptgis genes in the melanization group are higher than those in the control group. In terms of function, three of these five genes are related to arachidonic acid(AA) to some extent. This provides theoretical support for the hypothesis that nutritional regulation related to unsaturated fatty acids may be involved in the molecular mechanism of abnormal body color in C. semilaevis.
作者
赵娜
何晓旭
贾磊
朱春华
张博
Zhao Na;He Xiaoxu;Jia Lei;Zhu Chunhua;Zhang Bo(College of Fisheries,Guangdong Ocean University,Zhanjiang 524025,China;Tianjin Fisheries Research Institute,Tianjin 300457,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Tianjin Agricultural University,Tianjin 300384,China)
出处
《海洋学报》
CAS
CSCD
北大核心
2022年第2期57-66,共10页
基金
天津市自然科学基金(17JCQNJC15000)
国家海水鱼产业技术体系项目(CARS-47-Z01)
天津市水产产业技术体系项目(ITTFRS2017011)。
关键词
皮肤
半滑舌鳎
无眼侧黑化
花生四烯酸
转录组
skin
Cynoglossus semilaevis
hypermelanosis
arachidonic acid
transcriptome
作者简介
赵娜(1984-),女,河北省保定市人,副研究员,博士,研究方向为水生动物免疫及代谢调节。E-mail:penguinzn@163.com;通信作者:张博,副研究员,博士,研究方向为遗传育种与生物技术。E-mail:zb611273@163.com;通信作者:朱春华,教授,博士,研究方向为水产养殖学。E-mail:hu860025@163.com。
