摘要
【目的】研究地菍粗多糖对高脂饮食诱导肥胖小鼠脂代谢的影响。【方法】将小鼠随机分为6组:空白组、模型组、阳性组(0.025 mg/g奥利司他)及高、中、低剂量地菍粗多糖(1.2、0.6和0.3 mg/g)处理组,每组12只。空白组小鼠喂食普通维持饲料,其余各组小鼠喂食高脂饲料,空白组、模型组给予生理盐水,阳性组给予0.025 mg/g奥利司他,高、中、低剂量地菍粗多糖组分别给予1.2、0.6和0.3 mg/g地菍粗多糖,每天灌胃1次,连续给药30 d。给药结束,称量小鼠体重、附睾及肾脏周围脂肪和肝脏组织的重量;HE染色法观察脂肪和肝脏病理变化;生化法检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量及肝脏TC、TG含量;实时荧光定量PCR法测定肝脏中乙酰辅酶A羧化酶1(ACC1)基因相对表达量。【结果】与模型组相比,高、中剂量地菍粗多糖组小鼠体重、Lee’s指数、摄食量、肝脏及附睾和肾脏周围脂肪的重量均显著降低(P<0.05),低剂量地菍粗多糖组小鼠体重、脂肪重量均无显著变化(P>0.05),阳性组小鼠摄食量、肝脏重量均无显著变化(P>0.05);阳性组、高剂量地菍粗多糖组小鼠的脂肪指数均显著降低(P<0.05);阳性组、高剂量地菍粗多糖组小鼠血清TC、TG、LDL-C及肝脏TC、TG含量均显著降低(P<0.05),血清HDL-C含量差异不显著(P>0.05);中剂量地菍粗多糖组小鼠血清TC、TG及肝脏TC含量均显著降低(P<0.05)。阳性组及高、中、低剂量地菍粗多糖组小鼠脂肪细胞均不同程度变小,肝细胞内脂滴小空泡明显减少,且ACC1基因的相对表达量均显著下调(P<0.05),分别降低了20%、42%、15%和11%。【结论】地菍粗多糖可通过调节高脂饮食诱导肥胖小鼠的脂代谢水平,从而干预肥胖的发生。
【Objective】This study was aimed to investigate the effect of crude polysaccharide from Melastoma dodecandrum Lour.on lipid metabolism in obese mice induced by high-fat diet.【Method】The mice were randomly divided into 6 groups,including blank group,model group,positive group(0.025 mg/g orlistat),high,medium and low dose of crude polysaccharide from Melastoma dodecandrum Lour.groups(1.2,0.6 and 0.3 mg/g),with 12 mice in each group.The mice in blank group were fed with normal maintenance diet,the mice in the other groups were fed with high-fat diet,the mice in blank group and model group were given normal saline,the mice in positive group were given 0.025 mg/g orlistat,and the mice in high,medium and low dose groups were given 1.2,0.6 and 0.3 mg/g crude polysaccharides of Melastoma dodecandrum Lour.,once a day for 30 days.The weight of mice,epididymis,fat around kidney and liver tissuea were measured.The pathological changes of fat and liver were observed by HE staining.Serum total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C)and liver TC and TG were detected by biochemical method.The relative expression of acetyl CoA carboxylase 1(ACC1)gene in liver was determined by Real-time quantitative PCR.【Result】Compared with model group,the body weight,Lee’s index,food intake,the weight of liver,epididymis fat and kidney fat were significantly decreased in the high and medium dose groups(P<0.05).The body weight and fat weight of mice in low dose group had no significant change(P>0.05),the food intake and liver weight of mice in positive group had no significant change(P>0.05).The fat index of mice in positive and high dose groups was significantly decreased(P<0.05).The contents of serum TC,TG,LDL-C and liver TC,TG in positive and high dose groups were significantly decreased(P<0.05),but there was no significant difference of serum HDL-C(P>0.05).The contents of TC,TG in serum and TC in liver were significantly decreased in middle dose group(P<0.05).In positive group,high,medium and low dose groups,the adipocytes were reduced in varying degrees,and the lipid droplets and vacuoles in hepatocytes were significantly reduced(P<0.05),and the relative expression of ACC1 gene was significantly down regulated(P<0.05)by 20%,42%,15%and 11%,respectively.【Conclusion】The crude polysaccharide of Melastoma dodecandrum Lour.could intervene the occurrence of obesity by regulating the level of lipid metabolism in obese mice induced by high-fat diet.
作者
李丽
朱盼
郭兴兰
潘炳强
周敬凯
陈俊
LI Li;ZHU Pan;GUO Xinglan;PAN Bingqiang;ZHOU Jingkai;CHEN Jun(The Key Laboratory of Chinese Medicine Pharmacology of Colleges and Universities in Guangxi,College of Pharmacy,Guangxi University of Traditional Chinese Medicine,Nanning 530200,China)
出处
《中国畜牧兽医》
CAS
北大核心
2022年第1期122-130,共9页
China Animal Husbandry & Veterinary Medicine
基金
广西研究生教育创新计划(YCSY2020078)
广西一流学科建设开放课题(2019XK082)
广西中医药大学大学生科研训练课题(2020DXS11)
广西第八批特聘专家项目(壮瑶药质量标准研究,桂人才通字[2019]13号)
中药学广西一流学科建设项目(桂教科研[2018]12号)
广西民族药资源与应用工程研究中心(桂发改高技函[2020]2605号)
广西壮瑶药重点实验室(桂科基字[2014]32号)。
关键词
地菍
粗多糖
肥胖
脂代谢
Melastoma dodecandrum Lour.
crude polysaccharide
obesity
lipid metabolism
作者简介
李丽,E-mail:lilygxnn@163.com;通信作者:陈俊,E-mail:543767193@qq.com。
