摘要
为探究湖北半夏(Pinellia ternate)内生细菌的多样性、丰度及菌群功能,随机采集半夏植株样品,提取内生细菌基因组DNA,通过PCR扩增获得16S rDNA V3~V4片段。采用IlluminaMiSeq高通量测序技术分析半夏植株中的内生细菌菌群结构,共获得有效序列39537条和用于物种分类的OTU数681条,包括6门、10纲、23目、29科、45属、26种的细菌。菌落多样性分析表明,湖北半夏内生细菌在门水平上,优势菌群为Proteobacteria,科水平上,Mitochondria和Pseudomonadaceae为优势菌群。功能预测结果表明,湖北半夏内生细菌编码的大多数基因与代谢相关,其次是基因信息处理、环境信息处理。
In order to explore the diversity,abundance and flora function of endophytic bacteria in Pinellia ternata in Hubei,this study randomly collected samples of Pinellia ternata plants,extracted endophytic bacterial genomic DNA,and obtained 16 S rDNA V3~V4 fragments by PCR amplification.The IlluminaMiSeq high-throughput sequencing technology was used to analyze the endophytic bacterial flora structure in Pinellia ternata plants.A total of 39537 effective sequences and 681 OTUs for species classification were obtained,including 6 phylum,10 class,23 order,29 family,45 genus,26 species of bacteria.The analysis of colony diversity indicated that Proteobacteria was the dominant bacteria atthephylumlevel,Mitochondria and Pseudomonadaceae were the dominant bacteriaat the family level.Functional prediction results indicated that most of the genes encoded by endophytic bacteria were related to metabolism,followed by genetic information processing and environmental information processing.
作者
王帆帆
唐涛
郭杰
段媛媛
郭晓亮
游景茂
WANG Fan-fan;TANG Tao;GUO Jie;DUAN Yuan-yuan;GUO Xiao-liang;YOU Jing-mao(Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China)
出处
《湖北农业科学》
2021年第23期172-176,共5页
Hubei Agricultural Sciences
基金
湖北省重点研发计划项目(2020BCA059)
湖北省农业科技创新中心2020年重大科技研发项目(2020-620-000-002-04)
国家中药材产业技术体系(CARS-21)
湖北省重点研发项目(2020BBB135)。
作者简介
王帆帆(1994-),女,湖北恩施人,硕士,主要从事中药材病虫害绿色防控研究,(电话)0718-8417890(电子信箱)wff164229@163.com;通信作者:游景茂(1983-),男,副研究员,(电子信箱)jingmaoyou@126.com。
