摘要
景天三七(Sedum aizoon L.)根、茎、花、叶样品经粉碎后以甲醇为溶剂进行索氏提取,以芦丁为对照品,NaNO_(2)-A1(NO_(3))_(3)-NaOH作显色剂,在优选的最大吸收波长365 nm处检测,建立总黄酮测定的紫外分光光度法并对其含量进行测定。结果表明,在浓度8~40μg/g吸光度线性关系良好,回归方程为y=0.0175x+0.04(R^(2)=0.9999),景天三七不同部位低、中、高3个浓度芦丁添加回收率在92%~94%,RSD≤5%;景天三七根、茎、花、叶中总黄酮含量分别为(29.44±1.37)、(27.96±0.87)、(9.79±0.36)和(16.16±0.50)mg/g。研究建立了一种简单、准确的景天三七不同部位总黄酮的测定方法,为景天三七不同部位总黄酮检测及有针对性地开发利用提供科学依据。
After the root,stem,flower and leaf of Sedum aizoon L.were crushed,the soxhlet extraction was carried out with methanol as solvent.Then,the content of total flavonoids in different organs was determined by the developed ultraviolet spectrophotometry method at the optimal maximum absorption wavelength of 365 nm with rutin as reference substance and NaNO_(2)-A1(NO_(3))_(3)-NaOH as chromogenic agent.A good linear behaviors over the investigated concentration ranges of 8~40μg/g were observed with the regression equation of y=0.0175 x+0.04(R^(2)=0.9999)for the test.The recoveries of added rutin at low,medium and high concentration levels in different organs was 92%~94%,RSD≤5%.The contents of total flavonoids in roots,stems,flowers and leaves of Sedum aizoon L.were(29.44±1.37),(27.96±0.87),(9.79±0.36)and(16.16±0.50)mg/g,respectively.This study established a simple and accurate method for the determination of total flavonoids in different organs of Sedum aizoon L.,which provided a scientific basis for the detection and targeted development and utilization of the flavonoids in Sedum aizoon L.
作者
王立业
牛江秀
王真真
吴玉琦
马紫玉
王艳艳
于志斌
WANG Li-ye;NIU Jiang-xiu;WANG Zhen-zhen;WU Yu-qi;MA Zi-yu;WANG Yan-yan;YU Zhi-bin(College of Food and Drug,Luoyang Normal University,Luoyang 471934,Henan,China;China Chamber of Commerce for Import&Export of Medicine&Heahh Products,Beijing 100010,China)
出处
《湖北农业科学》
2021年第23期131-135,共5页
Hubei Agricultural Sciences
基金
洛阳师范学院横向科研项目(190726,191212)
河南省重点研发与推广专项(科技攻关)(202102110103)。
作者简介
王立业(1987-),男,河南洛阳人,讲师,博士,主要从事药物分析,(电话)0379-68618516(电子信箱)liye2009314@163.com;通信作者:于志斌(1979-),山东青岛人,硕士,主要从事中药进出口研究,(电子信箱)yuzhibin@cccmhpie.org.cn。
