摘要
目的探讨RS102895特异性拮抗CC趋化因子受体2(CCR2),对不同恶性程度前列腺癌细胞生物学行为的影响。方法体外培养非雄激素依赖性PC-3前列腺癌细胞和雄激素依赖性22RV1前列腺癌细胞,设立空白对照组、重组CC趋化因子配体2(rCCL2)处理组、rCCL2联合RS102895处理组。CCK-8法检测细胞增殖能力,Transwell^(TM)法检测细胞侵袭和迁移能力,实时定量PCR检测细胞KI-67抗原(ki67)与基质金属蛋白酶2(MMP2)的mRNA表达水平,Western blot法检测细胞ki67、MMP2的蛋白表达水平。结果与对照组相比,rCCL2处理能够显著增强PC-3细胞的增殖、侵袭及迁移能力,对22RV1细胞的增殖能力具有显著增强作用;同时,rCCL2处理可显著上调PC-3细胞中ki67和MMP2的mRNA及蛋白表达水平。RS102895处理后,可逆转rCCL2的以上作用。结论RS102895可通过特异性阻断PC-3前列腺癌细胞中CCL2/CCR2通路,下调ki67及MMP2的表达,抑制细胞的增殖、侵袭和迁移。
Objective To investigate the effect of RS102895,a specific C-C motif chemokine receptor 2(CCR2)antagonist,on the biological behavior of prostate cancer(PCa)cells with different degrees of malignancy.Methods Non-androgen-dependent prostate cancer cells PC-3 and androgen-dependent prostate cancer cells 22RV1 were cultured in vitro.A control group,a recombinant C-C motif chemokine ligand 2(rCCL2)treatment group,and a rCCL2 combined with RS102895 treatment group were established.Cell proliferation ability was detected by CCK-8 assay,cell invasion and migration abilities were detected by Transwell^(TM) assay,mRNA expressions of cell antigen KI-67(ki67)and matrix metalloproteinase 2(MMP2)were detected by real-time quantitative PCR,and protein expression levels of ki67 and MMP2 were detected by Western blotting.Results The proliferation,invasion,and migration abilities of PC-3 cells were significantly enhanced by rCCL2,and the proliferation ability of 22RV1 cells was significantly increased as well.Meanwhile,the mRNA and protein expression levels of ki67 and MMP2 in PC-3 cells were significantly up-regulated by rCCL2.After RS102895 treatment,the above effects of rCCL2 were reversed.Conclusion RS102895 can inhibit the proliferation,invasion,and migration of PC-3 prostate cancer cells by specifically blocking the CCL2/CCR2 pathway and down-regulating the expressions of ki67 and MMP2.
作者
王竞州
陈柯茹
李雪
杨冰琪
庞槐
王翠喆
张君
WANG Jingzhou;CHEN Keru;LI Xue;YANG Bingqi;PANG Huai;WANG Cuizhe;ZHANG Jun(Key laboratory of Xinjiang Endemic and Ethnic Diseases,School of Medicine,Shihezi University,Shihezi 832000,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2021年第9期781-787,共7页
Chinese Journal of Cellular and Molecular Immunology
基金
兵团国际合作项目(2016AH005)
国家自然科学基金(81760518)
石河子大学科研项目(ZZZC201817A)
石河子大学自主资助支持科研创新项目(ZZZC202017A)。
作者简介
王竞州(1994-),男,甘肃天水人,助教,硕士。Tel:18299080487,E-mail:neil_wjz@163.com;通讯作者:张君,E-mail:zhangjunyc@163.com。
