摘要
海洋微生物可以产生胞外活性氧(reactive oxygen species,ROS),为深海无光区提供了活性氧.而在实际海洋环境中,海洋微生物更倾向于以生物膜形式生长,但生物膜形态对菌体产ROS的影响鲜见报道.考察了海洋假交替单胞菌(Pseudoalteromonas sp.)GCY生物膜产ROS特性.结果表明,GCY生物膜胞外H_(2)O_(2)、O^(·-)_(2)以及·OH等产量均高于游离菌体,其中生物膜体系H_(2)O_(2)产量及O^(·-)_(2)产量最高可达到游离菌体体系的3.10倍和3.56倍.同时L-氨基酸氧化酶(LAAO)、脱氢酶活性(DHA)、电子传递系统活性(ETSA)均有所提高.以生物膜形式生长的GCY与游离态GCY相比,胞外聚合物组分中多糖和蛋白质含量均有提升,胞外聚合物(EPS)总量增加了149 mg·L^(-1),增强了细胞黏附性并减少了ROS对细胞造成的损伤.
Marine microorganisms,which can produce extracellular reactive oxygen species(ROS),are inferred as source of ROS for marine aphotic zones.However,in the actual marine environment,microorganisms have the tendency to form into biofilms,and the effect of biofilm on ROS production has rarely been reported.So ROS production characteristics of Pseudoalteromonas sp.GCY biofilm are investigated.According to the results,the extracellular H_(2)O_(2)、O^(·-)_(2) and ·OH production of GCY biofilm is higher than that of free cells.Extracellular H2O2 production and extracellular O^(·-)_(2) production are 3.10 times and 3.56 times higher than that of free cells,respectively.Meanwhile,the activities of L-amino acid oxidase(LAAO),dehydrogenase activity(DHA)and electron transport system activity(ETSA)are also increased.Compared with free cells,polysaccharide and protein contents in extracellular polymer substance(EPS)components are both increased.The total EPS production increases by 149 mg·L^(-1),which enhances cell adhesion and reduces ROS-induced damage to cells.
作者
吕菁萍
李泽龙
张鹤睿
王竞
LÜJingping;LI Zelong;ZHANG Herui;WANG Jing(School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China)
出处
《大连理工大学学报》
CAS
CSCD
北大核心
2021年第6期569-575,共7页
Journal of Dalian University of Technology
基金
国家自然科学基金资助项目(21876018).
关键词
活性氧
生物膜
假交替单胞菌
海洋环境
reactive oxygen species
biofilm
Pseudoalteromonas sp.
marine environment
作者简介
吕菁萍(1996-),女,硕士生,E-mail:2515280303@qq.com;王竞(1967-),男,教授,博士生导师,E-mail:jwang@dlut.edu.cn.
