摘要
FAR1/FHY3是由转座酶衍生的一类转录因子家族,它通过调控下游基因表达,进而在植物光响应、生长发育及逆境胁迫应答过程发挥重要作用,但当前有关FAR1/FHY3家族的研究主要集中在模式植物拟南芥(Arabidopsis thaliana)中。为了探究番茄(Lycopersicon esculentum) FAR1/FHY3基因家族的进化和功能,该研究通过全基因组分析鉴定到27个FAR1/FHY3基因,并利用生物信息学手段和荧光定量PCR技术(qRTPCR)对其展开分析。结果表明,该基因家族可分为7个亚家族,与拟南芥和玉米(Zea mays)具有相似分类,不同亚族之间基因结构存在显著差异;该家族成员在4、5和12号以外的所有染色体上均呈现不均衡分布,并且存在4个串联重复的基因簇;组织表达分析表明,个别亚族之间存在组织表达相似性,多数组织中均存在相对高表达基因,特别是第I亚族基因在果实发育及成熟过程特异高表达;qRT-PCR结果表明大多数成员能被NaCl、PEG6000胁迫诱导表达,而被ABA处理所抑制,特别是SlFAR1-22、SlFAR1-23和SlFAR1-25基因能够分别被PEG6000胁迫诱导上调表达28.06、14.25和9.09倍,暗示FAR1/FHY3家族在胁迫应答过程具有重要作用。该研究结果可为进一步解析该家族成员在番茄生长发育及胁迫应答过程中的功能机制奠定基础。
FAR1/FHY3 is a transcription factor family derived from transposase, which play crucial roles in plant light response, growth and development, and stress response processes through regulating the expression of downstream genes. However, the current studies on the FAR1/FHY3 gene family are mainly focus on the model plant Arabidopsis. In order to explore the evolution and function of the tomato FAR1/FHY3 gene family, this study identified 27 FAR1/FHY3 genes through genome-wide analysis, and further analysis were carried out by using bioinformatics methods and quantitative real-time PCR(qRT-PCR). The results demonstrated that these genes can be divided into 7 subfamilies, which are similar to Arabidopsis and corn, and there are significant differences in gene structure between different subfamilies. The members of this family are unevenly distributed on all chromosomes except chromosomes 4, 5 and 12, and there are four tandem repeat gene clusters. Tissue expression analysis indicates that there are tissue expression similarities between several individual subgroups, and relatively high expression genes exist in most tissues, especially subgroup I genes are relative high expression during fruit development and ripening processes. qRT-PCR results demonstrated that most of the members can be induced by NaCl and PEG6000 stress, but inhibited by ABA treatment, especially SlFAR1-22, SlFAR1-23 and SlFAR1-25 can be up-regulated by PEG6000 stress by 28.06, 14.25 and 9.09 times, respectively, suggesting that the tomato FAR1/FHY3 genes family plays an important role in PEG6000 stress response. The results of this study can lay the foundation for further analysis of the functional mechanism of this family members in the process of tomato growth and stress response.
作者
陈渝
邓洁
陈君愉
曾亭
余婷婷
黄千华
陈培超
刘庆
简伟
杨星勇
CHEN Yu;DENG Jie;CHEN Junyu;ZENG Ting;YU Tingting;HUANG Qianhua;CHEN Peichao;LIU Qing;JIAN Wei;YANG Xingyong(School of Life Sciences,Chongqing Normal University,Chongqing 401331,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2021年第10期1983-1995,共13页
Plant Physiology Journal
基金
重庆市自然科学基金面上项目(cstc2020jcyj-msxm X0774)
国家自然科学基金青年科学基金项目(32001845)
重庆市教委科学技术研究项目(KJQN202000520)
重庆师范大学博士启动基金(19XLB004)。
作者简介
通信作者:简伟,jianwei7956@163.com。
