摘要
目的探讨针刺对阿尔茨海默症小鼠海马CA1区树突结构及认知功能的影响。方法24只雄性快速老化SAMP8小鼠,随机分为模型组和针刺组,各12只;另选取12只雄性正常老化SAMR1小鼠为对照(对照组)。对照组和模型组小鼠不做处理,针刺组小鼠接受为期8周(隔日治疗一次)的针刺治疗,针刺部位为百会穴、肾俞穴、太溪穴。Morris水迷宫实验检测小鼠认知功能;取小鼠海马CA1区,Golgi染色观察锥体细胞树突结构;免疫组织化学染色法检测Aβ1-40和Tau表达;Western blot检测BDNF、SYN和MAP2蛋白表达水平。结果(1)与对照组比较,模型组水迷宫训练第5天时逃避潜伏期较高,而跨平台次数较低(P<0.05)。与模型组比较,针刺组水迷宫训练第5天时逃避潜伏期较低,而跨平台次数较高(P<0.05)。(2)与对照组比较,模型组基底树突长度、顶端树突长度、基底树突分数数目和顶端树突分支数目均较低(P<0.05)。与模型组比较,针刺组上述指标均较高(P<0.05)。(3)与对照组比较,模型组Aβ1-40和Tau表达的平均光密度值较高(P<0.05)。与模型组比较,针刺组上述指标较低(P<0.05)。(4)与对照组比较,模型组BDNF、SYN和MAP2蛋白表达水平均较低(P<0.05)。与模型组比较,针刺组上述指标均较高(P<0.05)。结论针刺百会、肾俞和太溪穴可能通过激活BDNF/SYN/MAP2信号通路,改善海马CA1区树突长度和分支数目从而改善快速老化SAMP8小鼠的认知功能。
Objective To explore the effect of acupuncture on the dendritic structure of the hippocampal CA1 area and cognitive functions in Alzheimer’s disease mice.Methods Twenty-four male rapidly aging SAMP8 mice were randomly divided into model and acupuncture groups with 12 mice each.Another 12 male normal aging SAMR1 mice were used as the control(control group).Mice in control and model groups were untreated.Mice in the acupuncture group received acupuncture treatment for 8 weeks(once every other day).The acupuncture sites were Baihui,Shenshu,and Taixi.A Morris water maze test was sued to assess mouse cognitive functions.After collecting the mouse hippocampal CA1 area,Golgi staining was used to observe the structure of pyramidal cell dendrites.Immunohistochemical staining was used to detect expression of Aβ_(1-40) and Tau.Western blot was used to measure BDNF,SYN,and MAP2 protein expression levels.Results(1)Compared with the control group,the model group had a higher escape latency on the 5^(th) day of water maze training,while the number of cross-platforms was lower(P<0.05).Compared with the model group,the acupuncture group had a lower escape latency on the 5^(th) day of water maze training,and a higher number of cross-platforms(P<0.05).(2)Compared with the control group,the lengths of basal and apical dendrites,and the numbers of basal dendrites and branches of apical dendrites were all lower in the model group(P<0.05).Compared with the model group,the above indicators were all higher in the acupuncture group(P<0.05).(3)Compared with the control group,the average optical density of Aβ_(1-40) and Tau expression was higher in the model group(P<0.05).Compared with the model group,the above indicators were lower in the acupuncture group(P<0.05).(4)Compared with the control group,the protein expression levels of BDNF,SYN,and MAP2 were lower in the model group(P<0.05).Compared with the model group,the above indexes were higher in the acupuncture group(P<0.05).Conclusions Acupuncture at Baihui,Shenshu and Taixi might improve the cognitive function of rapidly aging SAMP8 mice by activating BDNF/SYN/MAP2 signaling pathway and improving the length and number of dendritic branches in the CA1 region of hippocampus.
作者
曹育
安玉兰
张卓铭
翟春涛
武峻艳
CAO Yu;AN Yulan;ZHANG Zhuoming;ZHAI Chuntao;WU Junyan(Department of Massage,Third Clinical College,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China;Department of History of Traditional Chinese Medicine,Basic Medical College,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619;Department of Acupuncture and Moxibustion,Third Clinical College,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619;Department of Encephalopathy,First Clinical College,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第10期9-15,共7页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(青年科学基金)项目(81804174)。
关键词
针刺
阿尔茨海默症
认知
突触可塑性
树突
acupuncture
Alzheimer’s disease
cognition
synaptic plasticity
dendrites
作者简介
曹育(1976—),女,讲师,研究方向:针灸推拿学。E-mail:loophite@163.com;通信作者:武峻艳(1979—),女,博士,研究方向:中医针灸治疗脑病的基础与临床研究。E-mail:54caoyu@sohu.com。
