摘要
目的研究腹主动脉瘤和动脉粥样硬化患者菌群多样性差异。方法2018年12月至2019年6月于北京协和医院血管外科收集腹主动脉瘤和动脉粥样硬化患者新鲜粪便样本各20例,采用16S rDNA高通量测序技术检测两组患者肠道菌群物种组成及丰度变化,分析α和β多样性差异,并确定显著性差异菌属。结果两组间在菌群物种组成方面具有较大相似性,α多样性差异无统计学意义,β多样性两组间在某些菌群上仍有一定差异。LEfSe分析发现,腹主动脉瘤组明串珠菌科、瘤胃球菌科、魏斯氏菌属、粪杆菌属较动脉粥样硬化组为优势菌,而厚壁菌纲、Selenomonadales菌目、韦荣氏菌科含量减少,差异均具有统计学意义。结论腹主动脉瘤和动脉粥样硬化患者在肠道菌群多样性方面存在差异,具体差异仍有待扩大样本量并通过基础实验予以验证。
Objective To investigate the differences of gut microbiota between patients with abdominal aortic aneurysm and atherosclerosis.Methods From December 2018 to June 2019,20 fresh stool samples were collected respectively from the patients with abdominal aortic aneurysm and atherosclerosis treated at the Department of Vascular Surgery,Peking Union Medical College Hospital.The 16S rDNA high-throughput sequencing was employed to compare the composition,abundance,andαandβdiversities of gut microbiota between the two disease groups,and further determine the significantly differential genera.Results The two groups had great similarities in the composition of gut microbiota.There was no statistical difference inαdiversity.Althoughβdiversity did not have statistically significant difference,certain microbial taxa showed differences between the two groups.The LEfSe demonstrated that the abdominal aortic aneurysm group had higher relative abundance of Leuconostocaceae,Ruminococcaceae,Weissella,and Faecalibacterium while lower relative abundance of Firmicuteria,Selenomonadales,and Veillonellaceae.Conclusion The structure of gut microbiota has differences between patients with abdominal aortic aneurysm and atherosclerosis,and sample size should be enlarged to validate the results.
作者
吉磊
顾光超
陈思良
王威
任金锐
李方达
吴建强
杨丹
郑月宏
JI Lei;GU Guangchao;CHEN Siliang;WANG Wei;REN Jinrui;LI Fangda;WU Jianqiang;YANG Dan;ZHENG Yuehong(Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;Medical Research Center,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;Department of Computational Biology and Bioinformatics,Institute of Medicinal Plant Development,CAMS and PUMC,Beijing 100193,China)
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2021年第5期677-684,共8页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金重大项目(51890894)
国家自然科学基金面上项目(81770481)。
作者简介
通信作者:郑月宏,电话:010-69152502,电子邮件:yuehongzheng@yahoo.com。
