摘要
建立超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定醋延胡索药材中黄曲霉毒素B1、B2、G1、G2含量的方法。样品以70%甲醇溶液提取,用免疫亲和柱净化,采用多反应检测(MRM)模式进行定量分析。当进样体积为2μL时,黄曲霉毒素B1、B2、G1、G2的质量分别在0.42~20.80、0.14~7.00、0.47~23.69和0.24~11.80 pg范围内与定量离子的色谱峰面积线性关系良好,相关系数分别为0.9994、0.9990、0.9989和0.9985,检出限分别为0.003、0.005、0.018、0.017μg/kg。高、中、低3个加标浓度水平下,4种黄曲霉毒素的平均回收率分别为88.94%、76.82%、104.90%和93.22%,测定结果的相对标准偏差分别为6.61%、3.71%、2.25%和4.18%(n=6)。该方法适用于醋延胡索药材中黄曲霉毒素的同时测定。
A method of simultaneous determination of aflatoxin B1,B2,G1 and G2 in vinegar corydalis by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.The samples were extracted with 70%methanol solution,purified with an immunoaffinity column,and analyzed quantitatively by multi reaction detection(MRM)mode.When the injection volume was 2μL,the mass of aflatoxin B1,B2,G1 and G2 had a good linear relationship with the chromatographic peak area of quantitative ions in the range of 0.42-20.80,0.14-7.00,0.47-23.69 and 0.24-11.80 pg,respectively,the correlation coefficients were 0.9994,0.9990,0.9989 and 0.9985,and the detection limits were 0.003,0.005,0.018 and 0.017μg/kg,respectively.The average recoveries of the four aflatoxins were 88.94%,76.82%,104.90%and 93.22%at high,medium and low spiked concentrations,and the relative standard deviations of the determination results were 6.61%,3.71%,2.25%and 4.18%(n=6)for aflatoxin B1,B2,G1 and G2,respectively.The method is suitable for the simultaneous determination of aflatoxin in vinegar corydalis.
作者
谭丽盈
Tan Liying(NO.1 Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510400,China)
出处
《化学分析计量》
CAS
2021年第10期27-32,共6页
Chemical Analysis And Meterage
作者简介
通信作者:谭丽盈,本科,主管中药师,主要从事中药质量标准研究和中药鉴定研究工作。
