摘要
建立了使用分散固相萃取技术结合HPLC-MS/MS的方法,测定化妆品中36种抗感染类药物的测定方法。采用电喷雾离子源,正/负离子多反应检测,液相色谱-质谱/质谱仪分离,基质曲线外标法定量。结果表明,36种抗感染类药物为4~100 ng/mL(氯霉素线性范围10.00~1000.00 ng/mL)的质量浓度范围内线性良好,经过0.5酸化乙腈提取,分散固相萃取粉末(由400 mg无水硫酸镁(分析纯),100 mg十八弯基键合硅胶吸附剂(C18),400 mg氯化钠)净化,对于阴性样品加标量为1.00和10.00μg/g范围内的回收率均为85%~110%。该方法适应范围广、灵敏度高并且高效,能满足实际样品检测的需要。
A method of HPLC-MS/MS combined with the dSPE was established to determine 36 kinds of forbidden anti-infective residues in cosmetics.In this method,the samples were seperated by liquid chromatography-mass spectrometry/mass spectrometer equipped with electrospray ion source,by positive ion multi-reaction detection,and quantified through the external standard of matrix curve.By the experiment result,36 kinds of forbidden anti-infective pesticide residues in 4~100 ng/mL(chloramphenicol in 10.00~1000.00 ng/mL)had good linear concentration range,by acetonitrile contained 0.5 percent formic acid extraction,dispersive solid-phase extraction powder(by 400 mg of anhydrous magnesium sulfate(analysis),150 mg of eighteen curved base bonded silica gel adsorbents(C18),400 mg of Sodium chloride)purification.The average under concentration in 1.00 g/kg and 10.00 g/kg level of negative samples were all between 85 percent and 110 percent.In brief,this method was suitable for the detection of 36 kinds of forbidden anti-infective residues in cosmetics depended on its wider application range,higher sensitivity and higher efficiency.
作者
王庆龄
裘钧陶
詹越城
叶磊海
仰海清
何斌
盛丽娜
WANG Qingling;QIU Juntao;ZHAN Yuecheng;YE Leihai;YANG Haiqing;HE Bin;SHENG Li'na(Zhejiang Gongzheng Testing Center Inc.,Hangzhou,Zhejiang 310009,China;Zanyu Techology Group Co.,Ltd.,Hangzhou,Zhejiang 310009,China)
出处
《农产品加工》
2021年第18期43-47,53,共6页
Farm Products Processing
作者简介
王庆龄(1991—),女,硕士,工程师,研究方向为食品检测。
