摘要
建立HPLC法同时测定养心草中5种成分的含量并采用单因素和正交试验筛选最优提取工艺。结果表明,采用建立的HPLC含量测定方法可以同时准确测定5种成分的含量,杨梅苷、芦丁、槲皮苷、槲皮素、山柰酚分别在22~88μg/mL、5~20μg/mL、18~64μg/mL、0.32~1.28μg/mL、0.56~2.24μg/mL,与峰面积线性关系良好,加样回收率在95.61%~107.38%,回收率的RSD为1.37%~4.60%,均符合要求,最优提取工艺为50%甲醇90℃加热回流提取30 min。
To establish a HPLC method to simultaneously determine the content of 5 compounds in Sedum aizoon L.and use single fac⁃tor and orthogonal experiments to select the optimal extraction process.The results showed that the established HPLC method can be used to determine the content of five components accurately at the same time,the concentrations of myricetin,rutin,quercitrin,quer⁃cetin and kaempferol in 22~88μg/mL,5~20μg/mL,18~64μg/mL,0.32~1.28μg/mL,0.56~2.24μg/mL have a good linear relation⁃ship with the peak area,respectively;the range of recovery rate of 5 compounds was 95.61%~107.38%;and the RSD values was 1.37%~4.60%.The optimal extraction process was 50%methanol,90℃heating reflux extraction for 30 min.
作者
张艳
丁仿霞
张建永
ZHANG Yan;DING Fang-xia;ZHANG Jian-yong(Zunyi Medical University,Zunyi 593099,Guizhou,China)
出处
《湖北农业科学》
2021年第17期121-124,共4页
Hubei Agricultural Sciences
基金
贵州省中医药管理局项目(QZYY-2015-143)
贵州省遵义市科技计划项目[遵市科合社字(2017)14号]。
关键词
养心草
HPLC
含量测定
提取工艺
优化
Sedum aizoon L.
HPLC
content determination
extraction process
optimizati
作者简介
张艳(1989-),女,山东泰安人,讲师,硕士,主要从事中药质量控制研究,(电话)15772271507,(电子信息)zy0027@zmu.edu.cn;通信作者:张建永,男,副教授,博士,主要从事中药质量评价研究,(电子信箱)zhangjianyong2006@126.com。
