摘要
【目的】分析不同配方培养基及贮藏条件对大果型黄百香果花粉离体萌发效果的影响,为大果型黄百香果花粉活力鉴定、人工辅助授粉及杂交育种提供理论依据。【方法】采集花瓣刚好完全展开大果型黄百香果花朵的花粉,采用L_(25)(5^(4))正交试验设计,筛选适宜其离体培养的蔗糖、硼酸、硝酸钙浓度及pH;利用最适宜花粉离体培养基,开展花朵不同采样时间、花粉离体贮藏湿度和温度对花粉活力影响试验,确定适宜大果型黄百香果花粉的离体贮藏条件。【结果】正交试验分析结果表明,影响大果型黄百香果花粉萌发率的因素排序为硝酸钙>蔗糖>硼酸>pH,确定花粉离体萌发的最适培养基为100.00 g/L蔗糖+0.02 g/L硼酸+0.40 g/L硝酸钙,培养基pH 5.0。在最适培养基中,大果型黄百香果花粉的萌发率为85.55%;于11:30—17:30采集大果型黄百香果花粉的萌发率均在80.00%以上,其中,开花后3 h(16:30)花粉的活力最强,显著高于除13:30和15:30外的其他时间采集花粉的萌发率(P<0.05,下同)。花粉贮藏试验结果表明,平铺在硫磺纸上自然风干处理3~48 h的花粉萌发率均显著高于同时间段密封于离心管中常温贮藏和平铺在硫磺纸上置于干燥器中贮藏处理,其中在硫磺纸上自然风干贮藏48 h的花粉萌发率仍达34.28%;自然风干5 h后密封于离心管中4℃贮藏24 h的花粉萌发率为61.49%,显著高于自然风干5 h后密封于离心管中-20℃贮藏24 h的花粉萌发率(55.22%)。【结论】大果型黄百香果花粉离体萌发最适培养基为100.00 g/L蔗糖+0.02 g/L硼酸+0.40 g/L硝酸钙及pH 5.0;当天采集花朵的花粉自然风干5 h后密封于离心管4℃贮藏其萌发效果最佳。
【Objective】In order to provide theoretical basis for pollen viability identification,artificial pollination and cross breeding of large-fruited yellow passion fruit,the effects of different culture medium in vitro and storage conditions on pollen germination were analyzed.【Method】Pollens were collected from newly blooming flowers of large-fruited yellow passion fruit.The suitable concentrations of sucrose,boric acid,calcium nitrate and suitable pH for pollen in vitro culture were screened by L_(25)(5^(4))orthogonal design.The effects of sampling time of flowers,storage humidity and temperature on pollen viability were studied by using the most suitable pollen culture medium.The suitable storage condition for pollen of large-fruited yellow passion fruit was determined.【Result】The results of orthogonal test showed that the order of influencing factors on pollen germination rate of large-fruited yellow passion fruit was calcium nitrate>sucrose>boric acid>pH.The optimum medium for pollen germination in vitro was 100.00 g/L sucrose+0.02 g/L boric acid+0.40 g/L calcium nitrate,and the medium pH was 5.0.The pollen germination rate in the optimum medium was 85.55%.The pollen germination rate of large-fruited yellow passion fruit was more than 80.00%when the sampling time was from 11:30 to 17:30.The pollen viability was the highest in the third hour(16:30)after flowering,which was significantly higher than the germination rate of pollens collected at other times except 13:30 and 15:30(P<0.05,the same as below).The results of storage test showed that,the pollen germination rate of natural air drying on sulfur paper treatment for 3-48 hours was significantly higher than that of sealed centrifugation tube storage at room temperature and storage in desiccator on sulfur paper for the same time,and the pollen germination rate of 48 h storage was still 34.28%.The pollen germination rate was 61.49% after natural air drying for 5 hours and sealed in centrifugal tube at 4℃ for 24 hours,which was significantly higher than that(55.22%)after natural air drying for 5 hours and sealed in centrifuge tube at-20℃ for 24 hours.【Conclusion】The optimum medium for pollen germination in vitro of large-fruited yellow passion fruit is 100.00 g/L sucrose+0.02 g/L boric acid+0.40 g/L calcium nitrate,and pH is 5.0.The best storage condition is that the pollens collected on the day are dried naturally for 5 hours and sealed in centrifuge tube at 4℃.
作者
刘洁云
吴艳艳
牟海飞
温放
田青兰
韦毅刚
张英俊
符龙飞
彭嘉宇
黄伟华
LIU Jie-yun;WU Yan-yan;MOU Hai-fei;WEN Fang;TIAN Qing-lan;WEI Yi-gang;ZHANG Ying-jun;FU Long-fei;PENG Jia-yu;HUANG Wei-hua(Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;Guangxi Institute of Botany,Chinese Academy of Sciences,Guangxi Guilin 541006,China;Agricultural Resource and Environment Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China)
出处
《西南农业学报》
CSCD
北大核心
2021年第8期1699-1704,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(32060660)
广西特色作物试验站项目(桂TS2016010)
广西农业科学院稳定资助科研团队项目(桂农科2021YT089)
广西农业科学院基本科研业务专项(桂农科2020YM61)。
关键词
百香果
花粉
离体培养
培养基筛选
贮藏条件
Passion fruit
Pollen
In vitro culture
Culture medium screening
Storage condition
作者简介
刘洁云(1988-),湖南郴州人,硕士,助理研究员,主要从事热带果树栽培与遗传育种研究工作,E-mail:1064776742@qq.com;通讯作者:黄伟华(1981-),E-mail:627852887@qq.com。
