摘要
【目的】探究外源油菜素内酯对低温胁迫下番茄幼苗的影响.【方法】以卡焰番茄为材料,低温胁迫过程中喷施BR及ABA,测定番茄幼苗叶片电解质渗透率、MDA含量、蔗糖相关酶、抗氧化酶活性、光合参数等.【结果】胁迫48 h后,BR处理的番茄幼苗叶片蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)活性相比对照及同时施用BR和ABA的处理提高了1.5倍,胁迫72 h后BR处理的番茄幼苗CAT活性比其他两个处理提高了2倍以上,POD活性高了1.5倍,而SOD活性高了1.8倍.胁迫72 h后BR处理的番茄幼苗叶片中MDA含量比对照降低了2.6倍,比同时施用BR和ABA的处理低了1.6倍,电导率降低1倍以上.【结论】施用外源BR后番茄幼苗的抗氧化酶活性升高从而保护幼苗膜系统,SS、SPS活性升高调节渗透系统从而使幼苗更好的应对低温胁迫,ABA对BR的效果有抑制作用.可见,BR通过调节幼苗生理代谢以增加对低温的耐受性,缓解效果随着胁迫时间的增加而提高,在48 h时对促进番茄幼苗的蔗糖转化效果最好,在72 h时对番茄幼苗膜系统的保护效果最好.
【Objective】The study aimed to explore the effect of exogenous brassinolide on tomato seedlings under low temperature stress.【Method】The tomato cultivar Kayan was employed,whose seedlings was sprayed with inhibitors BR and ABA during the period of low-temperature stress,followed by the measurements of electrolyte permeability,MDA content,sucrose-related enzymes,antioxidant enzyme activities and photosynthetic parameters.【Result】After 48 hours of stress,the activities of sucrose synthase(SS)and sucrose phosphate synthase(SPS)in the leaves of tomato seedlings treated with BR were significantly increased by 1.5 times compared with the other treatment(i.e.,the control,and both BR and ABA).After 72 hours of stress,CAT activity,POD activity and SOD activity in the BR-treated seedlings were significantly increased by 2.0,1.5,and 1.8 times,respectively,compared with the other two treatments.For the 72 hours of stress,MDA content in the controlled leaves was 2.6 times higher than BR treatment,and that in the treatment with both BR and ABA was1.6 times higher than BR treatment,and the conductivity of BR treatment was decreased by more than 50%compared with the other two groups.【Conclusion】The application of exogenous BR to tomato seedlings could increase antioxidant enzyme activity of the leaves,and whereby protect their membrane system.The increased activities of SS and SPS could regulate osmotic system of the seedlings so that they could better cope with low temperature stress.ABA could inhibit the effectiveness of BR.Therefore,BR can improve the seeding's tolerance to low temperature by regulating their physiological metabolism,and the effectiveness increased with prolonged stress time.For the 48 hours of stress,the BR treatment could distinctly promote sucrose transformation of tomato seedlings,and for the 72 hours of stress,it could protect their membrane system.
作者
王光正
吕剑
毛娟
陈佰鸿
冯致
马宗桓
WANG Guangzheng;LYU Jian;MAO Juan;CHEN Baihong;FENG Zhi;MA Zonghuan(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)
出处
《甘肃农业大学学报》
CAS
CSCD
2021年第4期69-75,共7页
Journal of Gansu Agricultural University
基金
甘肃农业大学大学生科研训练计划(201912039)
国家自然科学基金项目(31660584)
甘肃省科技重大专项计划(17ZD2NA015).
关键词
番茄
低温细迫
油菜素内酯
生理指标
tomato
low temperature stress
brassinolide
physiological index
作者简介
第一作者:王光正,硕士研究生,E-mail:2435004270@qq.com;通信作者:冯致,副研究员,主要从事蔬菜设施栽培生理的研究,E-mail:Fengz@gsau.edu.cn;通信作者:马宗桓,讲师,主要从事果树生理及生物技术研究,E-mail:mazohu@163.com。
