摘要
目的:探讨1,25-二羟维生素D3[1,25(OH)_(2)D_(3)]对食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)细胞增殖、迁移和细胞周期的影响及其相关机制。方法:用不同浓度1,25(OH)_(2)D_(3)处理ESCC细胞TE-11、KYSE30、TE-1和KYSE510后,用CCK-8法检测细胞的增殖能力。再用浓度分别是0、0.1、0.15、0.2μmol/L的1,25(OH)_(2)D_(3)处理TE-11和KYSE30细胞,划痕愈合实验、流式细胞术分别检测细胞的迁移能力和细胞周期分布情况,WB法检测细胞中cyclin D1、P27、ERK和p-ERK蛋白的表达水平。结果:1,25(OH)_(2)D_(3)显著抑制TE-11和KYSE30细胞的增殖能力,其抑制程度呈时间依赖性和浓度依赖性。0.1和0.2μmol/L的1,25(OH)_(2)D_(3)处理48 h后,与空白对照组比较,TE-11和KYSE30细胞的迁移能力均显著降低(P<0.05或P<0.01),处于G0/G1期细胞显著增加(P<0.05或P<0.01),细胞中cyclin D1和p-ERK蛋白水平显著下调、P27蛋白水平明显上调(P<0.05或P<0.01)而ERK蛋白的表达无明显变化。结论:1,25(OH)_(2)D_(3)显著抑制ESCC细胞的增殖和迁移能力并阻滞细胞周期进程,其可能通过调控ERK信号通路而发挥作用。
Objective:To investigate the effects of 1,25(OH)_(2)D_(3)(1,25-dihydroxy vitamin D3)on the proliferation,migration and cell cycle of esophageal squamous cell carcinoma(ESCC)cells and its mechanism.Methods:Human ESCC cells(TE-1,KYSE30,TE-11 and KYSE510)were treated with different concentrations of 1,25(OH)_(2)D_(3),and the proliferation was detected by CCK-8.TE-11 and KYSE30 cells were treated with 1,25(OH)_(2)D_(3)at concentrations of 0,0.1,0.15,and 0.2μmol/L,respectively.Scratch healing test was used to detect cell migration ability,Flow cytometry was used to detect cell cycle distribution,and WB was used to detect the protein expressions of cyclin D1,P27,ERK and p-ERK.Results:1,25(OH)_(2)D_(3)significantly inhibited the proliferation of TE-11,KYSE30 cells in a concentration-and time-dependent manner(P<0.05 or P<0.01).As compared with control group,TE-11 and KYSE30 cells that treated with 0.1 and 0.2μmol/L 1,25(OH)_(2)D_(3)for 48 h exhibited significantly inhibited migration ability(P<0.05 or P<0.01),increased cell population at G0/G1 phase(P<0.05 or P<0.01),decreased protein expressions of cyclin D1 and p-ERK(P<0.05 or P<0.01),and increased expression of P27 protein(P<0.05 or P<0.01);however,there was no significant difference in total ERK level.Conclusion:1,25(OH)_(2)D_(3)can significantly inhibit the proliferation,migration and block the cell cycle of ESCC cells,which may be through the regulation of ERK signal pathway.
作者
熊蓉
熊黎
吴佳林
应凤
岳秋菊
胡欣
刘康
冯刚
XIONG Rong;XIONG Li;WU Jialin;YING Feng;YUE Qiuju;HU Xin;LIU Kang;FENG Gang(Institute of Tissue Engineering and Stem Cells,the Second Clinical Medical College of North Sichuan Medical College,the Second Clinical Medical College,North Sichuan Medical College,Nanchong 637000,Sichuan,China;School of Basic Medical Science,the Second Clinical Medical College,North Sichuan Medical College,Nanchong 637000,Sichuan,China;School of Clinical Medicine,the Second Clinical Medical College,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Department of Oncology,the Second Clinical Medical College,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2021年第7期696-701,共6页
Chinese Journal of Cancer Biotherapy
基金
四川省科技创新苗子工程培育项目(No.2020104)
四川省科技计划项目(No.2018SZ0377,No.2018JY0219)
南充市市校合作科研专项资金(No.18SXHZ0208,No.18SXHZ0366,No.18SXHZ0381)。
关键词
食管鳞状细胞癌
1
25-二羟维生素D_(3)
增殖
迁移
细胞周期
ERK通路
esophageal squamous cell carcinoma(ESCC)
1,25-dihydroxyvitamin D_(3)(1,25(OH)_(2)D_(3)
proliferation
migration
cell cycle
ERK signal pathway
作者简介
熊蓉(1993—),女,硕士生,主要从事食管癌的生物治疗研究,E-mail:xiongrong2019214@163.com;通信作者:刘康,博士,副研究员,硕士生导师,主要从事食管癌的基础及生物治疗研究,E-mail:liukang@nsmc.edu.cn。
