摘要
目的研究人类剪切蛋白(drosophila behavior human splicing,DBHS)家族基因,包括p5nrb、PSF、PSPC1基因,对食管鳞状细胞癌(以下简称食管磷癌)细胞系TE-8细胞系迁移、侵袭能力的影响。方法构建p54nrb、PSF、PSPC1基因过表达载体,通过脂质体法转染TE-8细胞,转染48 h后qRT-PCR和Western blotting检测各组细胞p54nrb、PSF、PSPC1在mRNA和蛋白质水平的表达。细胞划痕实验及覆盖有Matrigel的Transwell小室检测食管鳞癌细胞的迁移、侵袭能力。结果过表达p54nrb、PSF、PSPC1后,qRT-PCR和Western blotting检测证实食管鳞癌TE-8细胞中上述基因表达在mRNA水平和蛋白质水平均明显上调,细胞划痕实验及Transwell小室实验证实食管鳞癌TE-8细胞过表达p54nrb、PSF、PSPC1后,其迁移、侵袭能力增强。结论过表达DBHS家族基因可促进食管鳞癌细胞的迁移侵袭。
Objective To investigate the effects of drosophila behavior human splicing（DBHS） family members, including p54nrb, PSF and PSPCI, on regulation of migration and invasion in human esophageal squamous cell carcinoma （ESCC） cell line TE-8. Methods After transfeetion with p54nrb, PSF and PSPC1 plasmids, the expression of mRNA and protein in human ESCC cell line TE-8 were determined by qRT-PCR and Western blotting, respectively. Wound healing and Matrigel invasion test were used to detect the effects of p54nrb, PSF and PSPC1 overexpression on migration and invasion of the cells, respectively. Results After 48 h of transfection with p54nrb, PSF and PSPC1 plasmids, the expression of relatives p54nrb, PSF and PSPC1 mRNA and protein levels were increased （ P 〈 0. 05 ）, respectiveIy. The migration rate was measured by wound healing after overexpression of p54nrb, PSF and PSPC1, was promoted significantly （ P 〈 0.05 ）. Matrigel invasion of the cells were significantly increased after p54nrb, PSF and PSPC1 plasmids transfection （P 〈 0. 05）. Conclusion Overexpression of DBHS family members could promote both migration and invasion of ESCC cell line.
出处
《首都医科大学学报》
CAS
北大核心
2016年第1期6-11,共6页
Journal of Capital Medical University
基金
国家自然科学基金(81302160
81272447)
国家消化系统疾病临床医学研究中心基金(2015BAI13B09)
北京市自然科学基金(7152043)~~
关键词
食管鳞癌
人类剪切蛋白
p54nrb
PSF
PSPC1
侵袭
迁移
esophageal squamous cell carcinoma
drosophila behavior human splicing （DBHS）
p54nrb
PSF
PSPCI
invasion
migra-tion
