摘要
目的建立测定射麻口服液中黄芩苷含量的高效液相色谱法。方法色谱柱为Waters SunFire C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.2%磷酸溶液(47∶53,V/V),流速为1.0 m L/min,柱温为30℃,检测波长为280 nm,进样量为10μL。结果黄芩苷进样量在0.041 91~0.838 20μg范围内与峰面积线性关系良好(r=0.999 9,n=5);精密度、稳定性、重复性试验的RSD分别为0.63%,0.58%,0.84%(n=5);平均加样回收率为98.51%,RSD为2.19%(n=6)。结论所建立的高效液相色谱法操作简便,重复性好,准确度高,可用于射麻口服液中黄芩苷的含量测定。
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of baicalin in Shema Oral Liquid.Methods The chromatographic column was Waters SunFire C18 column(250 mm×4.6 mm,5μm),the mobile phase was methanol-0.2%phosphoric acid solution(47∶53,V/V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 280 nm,and the injection volume was 10μL.Results The linear range of baicalin was 0.04191-0.83820μg(r=0.9999,n=5).The RSDs of precision,stability and repeatability tests were 0.63%,0.58%and 0.84%(n=5),respectively.The average recovery of baicalin was 98.51%with RSD of 2.19%(n=6).Conclusion The method is simple,reproducible and accurate,which can be used for the content determination of baicalin in Shema Oral Liquid.
作者
杨巧虹
邓开英
申丽莎
YANG Qiaohong;DENG Kaiying;SHEN Lisha(Chongqing Quality Testing and Inspection Center for Medical Devices,Chongqing,China 401147;Chongqing Institute for Food and Drug Control·Chongqing Engineering Center for Pharmaceutical Process and Quality Control,Chongqing,China 401121;Chongqing Academy of Chinese Materia Medica,Chongqing,China 400061)
出处
《中国药业》
CAS
2021年第15期65-67,共3页
China Pharmaceuticals
作者简介
第一作者:杨巧虹,女,大学本科,副主任药师、副编审,研究方向为医疗器械检验检测实验室质量监督管理、药品质量控制方法研究论文的设计与编撰,(电话)023-67786713(电子信箱)87831702@qq.com;通信作者:申丽莎,女,大学本科,工程师,研究方向为中药质量控制,(电子信箱)shenlisa@163.com。
