摘要
分析新疆传统特色发酵食品——奶疙瘩的菌相组成,研究奶疙瘩发酵益生菌对秀丽隐杆线虫衰老相关生理功能和基因转录的影响,阐明其抗衰老作用机制。利用高通量测序方法和生物统计学方法分析新疆5种代表性奶疙瘩的菌相组成及规律,结果显示:优势细菌菌群主要是乳杆菌属、链球菌属、假单胞菌属、乳球菌属。在RS1.1样品外的4个样品中,乳酸菌属均为优势菌,从中分离获得具有延寿功效的耐酸、耐胆盐益生菌鼠李糖乳杆菌R4。转录组测序结果显示:鼠李糖乳杆菌R4对胰岛素信号通路中关键酶编码基因具有激活作用,可显著提高基因sod-3、hsp-16.2、ctl-1和daf-16的转录水平,而基因daf-2、sir-2.1的mRNA水平明显降低,表明R4通过胰岛素信号通路发挥作用,从而延长线虫的寿命。
The aim of the study was to analyze the bacteria composition of the traditional fermented cheese-milk knot in Xinjiang and the effect of the fermented probiotics on the physiological function and gene expression associated with senescence of Caenorhabditis elegans to explore the mechanism of its anti-aging effect.High throughput sequencing method and biostatistics method were used to analyze the composition and regulation information and the results showed that the dominant bacterial groups were mainly Lactobacillus,Pseudomonas,Streptococcus,Lactococcus and Lactobacillus is the dominant bacterium in the other four samples except RS1.1 samples.Lactobacillus rhamnosus R4,an acid-resistant and bile tolerant probiotic with life-extending effect,was isolated.Transcriptome sequencing showed that L.rhamnosus R4 stimulated the key enzyme coding genes in the insulin signaling pathway.L.rhamnosus R4 significantly increased the transcription levels of sod-3,hsp-16.2,ctl-1,and daf-16,while the expression levels of daf-2 and sir-2.1 were significantly down-regulated,suggesting that their effects on longevity of nematodes depend on insulin signaling pathway.
作者
李墨钊
热米拉·阿扎提
郑晓冬
周文文
Li Mozhao;AZAT Ramila;Zheng Xiaodong;Zhou Wenwen(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第7期149-156,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
国家重点研发计划项目(2018YFA0903200)
浙江省自然科学基金项目(LY20C200005)。
关键词
新疆奶疙瘩
秀丽隐杆线虫
菌相分析
抗衰老
胰岛素通路
Xinjiang milk knot
Caenorhabditis elegans
microflora analysis
anti-aging effect
insulin signaling pathway
作者简介
李墨钊(1999-),女,本科生;通信作者:周文文,E-mail:vivianzhou11@zju.edu.cn。
