摘要
采用钙透明圈法从西藏地区牦牛粪和乳源中分离、筛选益生菌,通过形态观察及分子生物学技术进行菌种鉴定,并对其生长特性、产酸能力、耐胆盐、酸、人工胃液能力及抑制大肠杆菌能力进行分析。结果表明,共分离、筛选出25株乳酸菌,经鉴定7株为益生菌,分别为1株植物乳杆菌(Lactobacillus plantarum),2株鼠李糖乳杆菌(Lactobacillus rhamnosus)和4株副干酪乳杆菌(Lactobacillus paracaseium)。其中植物乳杆菌B2具有良好的生长优势、产酸能力(发酵终pH值在4左右)、耐酸(pH值为4)、耐胆盐(0.6%)、耐人工胃液能力(存活率为57.4%),且抑制大肠杆菌效果较好(抑菌圈直径为22.0 mm)。
Probiotics were isolated and screened from yak dung and milk in Tibet by calcium transparent circle method,and identified through morphological observation and molecular biology techniques.Their growth characteristics,acid production capacity,bile salt,acid,artificial gastric juice resistance and inhibit ability to Escherichia coli were analyzed.The results showed that a total of 25 strains of lactic acid bacteria were isolated and screened,among them,7 strains were identified as probiotics,which were 1 strain of Lactobacillus plantarum,2 strain of Lactobacillus rhamnosus and 4 strains of Lactobacillus paracaseium.Among them,L.plantarum B2 had good growth advantages,acid production capacity(the final pH value of fermentation was about 4),acid resistance(pH 4),bile salt resistance(0.6%),artificial gastric juice resistance(survival rate 57.4%),and inhibitory effect on E.coli(inhibition zone diameter 22.0 mm).
作者
王帅静
李啸
刘玲彦
谈亚丽
肖泽涛
王庆宇
裴宇鹏
冯雪娜
WANG Shuaijing;LI Xiao;LIU Lingyan;TAN Yali;XIAO Zetao;WANG Qingyu;PEI Yupeng;FENG Xuena(China Light Industry Key Laboratory of Yeast Function,College of Biological&Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,China;Hubei Provincial Key Laboratory of Yeast Function,Angel Yeast Co.,Ltd.,Yichang 443003,China;Hubei Provincial Key Laboratory of Yeast Function,Angel Biological Group Co.,Ltd.,Yichang 443003,China)
出处
《中国酿造》
CAS
北大核心
2021年第7期43-48,共6页
China Brewing
基金
宜昌市科技专项(A16-101)。
关键词
益生菌
筛选
鉴定
生长特性
耐受性
抑菌性能
probiotics
screening
identification
growth characteristic
tolerance
antibacterial activity
作者简介
王帅静(1995-),女,硕士研究生,研究方向为潜在工业化益生菌的分离筛选;通讯作者:李啸(1969-),男,教授,博士,研究方向为微生物反应过程的优化与控制。
