摘要
【目的】建立一种新会陈皮陈化方式的判别方法。【方法】以不同来源、不同陈化年份、不同处理方式等49批陈皮样品为研究对象。根据陈皮高温加速陈化会发生Maillard反应的特点,建立灵敏度高、重复性好的高效液相色谱(HPLC)-QDa质谱检测器联用测定5-羟甲基糠醛含量的方法;制定可以有效判别出高温加速陈化陈皮的5-羟甲基糠醛含量限度;在此基础上,引入色差分析仪对陈皮外观色泽进行客观的数据量化,分析非高温加速样品的总色差值ΔE变化范围,制定可以有效判别出煮茶染色加速陈皮的ΔE值限度。【结果】不同来源陈皮的5-羟甲基糠醛含量存在明显差异,其中,自然陈化陈皮5-羟甲基糠醛含量均<0.25 mg·g^(-1),高温加速陈化陈皮的5-羟甲基糠醛含量均≥0.25 mg·g^(-1),而煮茶染色处理样品的含量也小于0.25 mg·g^(-1)。结合总色度值ΔE进一步研究发现,自然陈化陈皮的ΔE值均>68,而煮茶染色加速陈皮的ΔE值均≤68。【结论】以双指标5-羟甲基糠醛含量≥0.25 mg·g^(-1)、ΔE≤68可作为判定加速陈化陈皮的依据。该判定方法同时控制外观色泽及含量限度,可以准确、客观地对陈皮陈化方式进行判别。
Objective To establish a method for distinguishing the aging mode of Pericarpium Citri Reticulatae from Xinhui.Methods Forty-nine batches of Xinhui Pericarpium Citri Reticulatae with different sources,different aging years and different treatments were set up as object of study.Firstly,a high perfrmance liquid chrmatgraphy(HPLC)-QDa mass detector method was developed for the highly selective and sensitive determination of 5-hydroxymethylfurfura content based on high temperature catalyzed effect on Maillard reaction in Pericarpium Citri Reticulatae.The limitation standards of 5-hydroxymethylfurfura content was established as discrimination method for high temperature accelerated aging Pericarpium Citri Reticulatae.On this basis,colorimeter was used to quantify the appearance color of Pericarpium Citri Reticulatae to analyze range of chromatic valueΔE on non-high temperature accelerated samples,and then the limited standards of theΔE value was established for tea water dyeing accelerated aging Pericarpium Citri Reticulatae.Results There were significant differences in 5-hydroxymethylfurfura content among different sources samples.5-hydroxymethylfurfura content<0.25 mg·g-1 in naturally aged Pericarpium Citri Reticulatae samples,≥0.25 mg·g-1 in high temperature accelerated aging Pericarpium Citri Reticulatae,and<0.25 mg·g-1 in tea water dyeing accelerated aging Pericarpium Citri Reticulatae.Combined withΔE values,it was found thatΔE>68 in naturally aged Pericarpium Citri Reticulatae samples,whileΔE≤68 in tea water dyeing accelerated aging Pericarpium Citri Reticulatae.Conclusion In this study,the two parameters(5-hydroxymethylfurfura content≥0.25 mg·g^(-1)andΔE value≤68)was used to distinguish the accelerated aging of Xinhui Pericarpium Citri Reticulatae.The method determination method is effective for simultaneously controlling appearance color and content limitation,for accurately and objectively distinguishing the aging mode of Xinhui Pericarpium Citri Reticulatae.
作者
陈明权
商雪莹
张怀
何风雷
覃仁安
CHEN Ming-Quan;SHANG Xue-Ying;ZHANG Huai;HE Feng-Lei;QIN Ren-An(Guangzhou Baiyunshan Pharmaceutical Co.Chenliji,Guangzhou 510288 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2021年第7期1454-1461,共8页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广州市科技计划项目珠江科技新星专题(编号:201710010122)。
关键词
新会陈皮
5-羟甲基糠醛
外观色差
加速陈化
Xinhui Pericarpium Citri Reticulatae
5-hydroxymethylfurfura
appearance color
accelerated aging
作者简介
陈明权(1988-),男,硕士,工程师,E-mail:g729cmq@126.com;通讯作者:张怀(1982-),男,硕士,工程师,E-mail:85739378@qq.com。
