摘要
为确定从蝙蝠葛根中提取生物碱的最佳工艺,以蝙蝠葛根为材料、乙醇溶液为提取剂,利用超声波辅助提取蝙蝠葛根生物碱。在超声功率175 W的条件下,通过单因素试验考察乙醇浓度、超声温度、超声时间、料液比对超声提取蝙蝠葛生物碱提取量的影响。应用Box-Benhnken中心组合方法进行4因素3水平设计,以蝙蝠葛根生物碱提取量为响应值,采用响应面法优化蝙蝠葛根生物碱的提取工艺。结果表明,超声波辅助提取蝙蝠葛根中生物碱的最佳工艺为料液比1∶40(g/mL)、乙醇浓度70%、超声温度40℃、超声时间2 h,在此工艺条件下,蝙蝠葛根生物碱的提取量为9.067 mg/g。该工艺条件切实可行,可用于控制和预测蝙蝠葛根中生物碱的提取。
The extraction of alkaloids from the roots of Menispermum dauricum was carried out under ultrasonication using Menispermum dauricum roots as the target material and ethanol solution as the extractant.In order to determine the best process for extracting alkaloids from the roots of Menispermum dauricum,under the condition of ultrasonic power of 175 W,the effects of ethanol concentration, ultrasonic temperature, ultrasonic time, and material-to-liquid ratio on the amount of alkaloids extracted by ultrasonic extraction were investigated by single-factor experiment.The Box-Benhnken central combination method was used to carry out a 4-factor 3-level design, with the extraction amount of the alkaloids of Menispermum dauricum root as the response value, and the response surface method was used to optimize the extraction process of the alkaloids of Menispermum dauricum root.The results showed that the best process for ultrasonic-assisted extraction of alkaloids from Menispermum dauricum root is 1∶40(g/mL) ratio of material to liquid, 70% ethanol concentration, ultrasonic temperature of 40 ℃,ultrasonic time of 2 h.With this process condition, the extraction amount of alkaloids from Menispermum dauricum root is 9.067 mg/g, showing high potential for the feasible and reliable extraction of alkaloids from Menispermum dauricum roots.
作者
岑忠用
苏江
高丽霞
严军
黄喜苗
CEN Zhong-yong;SU Jiang;GAO Li-xia;YAN Jun;HUANG Xi-miao(College of Chemistry and Bioengineering,Hechi University,Yizhou 546300,China;School of Chemistry and Chemical and Engineering,Guangxi University for Nationalities,Nanning 530008,China)
出处
《化学试剂》
CAS
北大核心
2021年第7期986-991,共6页
Chemical Reagents
基金
广西高校重点实验室微生物及植物资源开发利用实验室资助项目(桂教科研[2016]6号)
河池学院校级重点实验室项目(校政发(2016)91号)。
关键词
蝙蝠葛
超声波
生物碱
响应面优化
提取工艺
Menispermum dauricum
ultrasonic
alkaloids
response surface methodology
extraction process
作者简介
岑忠用(1977-),男,壮族,广西田东人,硕士,副教授,主要从事药用资源开发与利用、植物组织培养及生理生化研究工作,E-mail:zhongyong20@163.com。
