摘要
为了建立快速、准确的甘蓝黑腐病苗期抗性鉴定方法,以田间对黑腐病抗感差异表现明显的5个供试材料作为鉴别寄主,喷雾接种黑腐病菌株,设置1叶1心、2叶1心、3叶1心、4叶1心、5叶1心等5个接种苗龄,1×10^(5)、1×10^(6)、1×10^(8)、1×10^(10)CFU·mL^(-1)等4种菌液接种浓度,26℃/19℃、28℃/19℃、30℃/19℃等3种培养温度,接种后0、12、24、36、48 h等5个保湿时间及接种后7、9、12、16 d等4个时间分别调查发病症状,确定影响抗性鉴定方法的关键因子。结果表明,上述处理组合能反映5个寄主材料的抗感特性,且高感与高抗材料的病情指数差值最大的分别为73.61、74.77和72.22。当甘蓝幼苗生长至3叶1心期,喷雾法接种浓度为1×10^(8)CFU·mL^(-1)菌液,接种后90%湿度下保湿36 h,在14 h光照、温度26℃/19℃培养下诱导病害发生,第12天调查幼苗发病程度,能够鉴定甘蓝材料的抗病性。
In order to establish a rapid and accurate method for identification of resistance to black rot in cabbage at the seedling stage,we used 5 materials with significant differences in resistance to black rot in the field as identification hosts,sprayed inoculation with black rot strains,set 5 inoculation seedling ages(one leaf and one heart,two leaves and one heart,three leaves and one heart,four leaves and one heart,five leaves and one heart),4 kinds of inoculation concentrations(1×10^(5)CFU·mL^(-1),1×106 CFU·mL^(-1),1×108 CFU·mL^(-1),1×1010 CFU·mL^(-1)),3 culture temperature treatments(26℃/19℃,28℃/19℃,30℃/19℃),5 moisturizing times(0 h,12 h,24 h,36 h,48 h)and 4 investigation time(7 d,9 d,12 d,16 d)to determine the key factors of the identification method.The results show that the above treatment combination can reflect the anti-susceptibility characteristics of the five host materials,and the difference between the disease indices of the high-sensitivity and high-resistance materials is 73.61,74.77 and 72.22,respectively.When the seedlings grew to the three leaves and one heart stage,the bacterial suspension with a concentration of 1×10^(8)CFU·mL^(-1),was inoculated by spraying method,moisturized at 90%humidity for 36 hours after inoculation,and the disease was induced under 14 h light and 26℃/19℃,the incidence of seedlings was investigated on the 12th day,the disease resistance of cabbage material could be identified.
作者
张鑫鑫
张恩慧
吴云锋
许忠民
蔡美杰
孙庆国
ZHANG Xinxin;ZHANG Enhui;WU Yunfeng;XU Zhongmin;CAI Meijie;SUN Qingguo(State Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China;College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《中国瓜菜》
CAS
北大核心
2021年第6期39-46,共8页
China Cucurbits And Vegetables
基金
国家“十三五”重点研发计划项目(甘蓝优质多抗适应性强新品种培育,2017YFD0101804)。
关键词
甘蓝
黑腐病
抗病性
苗期
鉴定方法
Cabbage
Black rot
Resistance
Seedling stage
Identification method
作者简介
张鑫鑫,女,在读硕士研究生,主要从事甘蓝育种与生物技术研究。E-mail:xinxinz1996@163.com;通信作者:张恩慧,男,教授,主要从事甘蓝类蔬菜育种与生物技术研究。E-mail:Ganlan606@126.com。
