摘要
采用单因素和正交试验法,研究野生古巴栓孔菌(Trametes cubensis)菌丝的最佳培养条件。单因素试验结果表明,古巴栓孔菌菌丝的最适碳源、氮源分别为葡萄糖、酵母浸粉,碳源、氮源的最适添加量分别为5 g/L,3 g/L,最适培养温度为35℃,培养基初始pH对古巴栓孔菌菌丝的生长影响不显著。正交试验结果表明,影响古巴栓孔菌菌丝体生长的因素从大到小依次为:碳源>温度>氮源。最佳培养基配方为:葡萄糖15 g/L,酵母浸粉3 g/L,硫酸镁0.5 g/L,磷酸二氢钾1 g/L,VB10.01 g/L,琼脂18 g/L,pH自然,36℃培养。
In this experiment,a single factor and orthogonal test methods were used to study the optimal culture conditions of a wild Trametes cubensis hyphae.The single factor test results showed that the optimal carbon source and nitrogen source for the mycelium of Trametes cubensis were glucose and yeast extract powder,and the optimal addition amount of carbon source and nitrogen source were 5 g/L,3 g/L.The optimum culture temperature was 35℃,and the initial pH of the medium had no significant effect on the growth of the hyphae of Trametes Cubensis.Orthogonal test results showed that the order of the effects on the growth of the mycelium of Trametes Cubensis was:carbon source>temperature>nitrogen source.The results of orthogonal test showed that the optimum medium was glucose 15 g/L,yeast extract 3 g/L,magnesium sulfate 0.5 g/L,monopotassium phosphate 1 g/L,VB10.01 g/L,Agar 18 g/L,natural pH,culture temperature 36℃.
作者
史维丽
班立桐
黄亮
杨红澎
孙宁
王玉
李奕葶
张怡
Shi Weili;Ban Litong;Huang Liang;Yang Hongpeng;Sun Ning;Wang Yu;Li Yiting;Zhang Yi(College of Agronomy and Resource Environment,Tianjin Agricultural University,Tianjin 300392,China)
出处
《天津农学院学报》
CAS
2021年第2期31-35,共5页
Journal of Tianjin Agricultural University
基金
天津市蔬菜产业技术体系创新团队项目(ITTVRS2017015)。
关键词
古巴栓孔菌
菌丝生长
温度
碳氮源
正交试验
Trametes cubensis
mycelial growth
temperature
carbon and nitrogen sources
orthogonal test
作者简介
史维丽(1994-),女,硕士在读,研究方向为作物栽培学与耕作学。E-mail:975576049@qq.com;通信作者:班立桐(1972-),男,教授,学士,主要从事食用菌栽培研究。E-mail:banlitong@126.com。
