摘要
以特丁基对苯二酚(TBHQ)为阳性对照,通过测定核桃虾青素复合油(纯核桃油+2%虾青素油)对DPPH自由基(DPPH·)、羟自由基(·OH)、超氧自由基(O_(2)^(-)·)清除能力和还原能力以及Fe^(2+)螯合能力,探讨复合油的体外抗氧化活性。结果表明:核桃虾青素复合油对DPPH·、O_(2)^(-)·、·OH的清除能力,还原能力和Fe^(2+)螯合能力随着核桃虾青素复合油质量浓度的增加而升高,且对DPPH·、O_(2)^(-)·、·OH的清除能力均强于纯核桃油,对DPPH·、O_(2)^(-)·、·OH的半抑制质量浓度(IC50)分别为8.25、0.81、1.30 mg/mL;还原能力方面表现为添加TBHQ的核桃油>核桃虾青素复合油>纯核桃油;核桃虾青素复合油的Fe^(2+)螯合能力明显强于纯核桃油和添加TBHQ的核桃油。研究表明核桃虾青素复合油较纯核桃油的体外抗氧化活性有所增强。
The in vitro antioxidant activity of walnut astaxanthin oil blend(walnut oil+2%astaxanthin oil)was studied by determining the scavenging capacity of DPPH·,hydroxyl radical(·OH)and superoxide anion radical(O_(2)^(-)·),reducing capcity and Fe^(2+)chelating capacity of walnut astaxanthin oil blend with tert-butylhydroquinone(TBHQ)as positive control.The results showed that scavenging capacity of walnut astaxanthin oil blend on DPPH·,O_(2)^(-)·and·OH,reducing capacity and Fe^(2+)chelating capacity increased with the increase of walnut astaxanthin oil blend mass concentration,and its scavenging capacity on DPPH·,O_(2)^(-)·,·OH were superior to the pure walnut oil.The half inhibitory mass concentration of walnut astaxanthin oil blend on DPPH·,O_(2)^(-)·,and·OH were 8.25,0.81,1.30 mg/mL,respectively.The reducing capacity of walnut oil+TBHQ was the strongest,followed by walnut astaxanthin oil blend and pure walnut oil.The walnut astaxanthin oil blend showed the strongest Fe^(2+)chelating capacity,followed by pure walnut oil and walnut oil+TBHQ.The results indicated that the antioxidant activity of walnut astaxanthin oil blend was higher than that of pure walnut oil in vitro.
作者
邢海亮
余旭亚
耿树香
宁德鲁
马婷
韩本勇
XING Hailiang;YU Xuya;GENG Shuxiang;NING Delu;MA Ting;HAN Benyong(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Yunnan Academy of Forestry and Grassland Sciences,Kunming 650204,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第5期48-52,共5页
China Oils and Fats
基金
云南省重大科技专项计划(2018ZG003)
国家自然科学基金(21766012)。
关键词
虾青素油
核桃油
核桃虾青素复合油
抗氧化活性
astaxanthin oil
walnut oil
walnut astaxanthin oil blend
antioxidant activity
作者简介
邢海亮(1995),男,硕士研究生,研究方向为微藻资源开发.E-mail:450442132@qq.com;通信作者:韩本勇,副教授,博士.E-mail:Xmcx668@126.com。
