摘要
目的以脊髓组织工程中的种子细胞和生物支架存在的问题为切入点,综述近期实验研究进展。方法检索建库至2021年3月中国知网、万方、PubMed和Web of Science数据库中的相关文献,并对种子细胞、生物支架和种子细胞-生物支架复合的问题及进展进行综述。结果种子细胞的问题在于致癌性、免疫排斥、伦理道德和移植后低存活率与分化率,现研究多集中于探索新细胞种类、基因转染、多细胞共移植和移植前预处理。生物支架的问题在于单一材料选择无法同时满足不同需求,且传统工艺也无法更好地模拟脊髓内部结构,因此,新型复合材料与新工艺成为研究热点。种子细胞-生物支架复合的核心问题在于不同种子细胞与生物支架复合的效果不一,现研究多致力于持续探索适宜的新复合模式,并尝试引入生物制剂等因素。结论脊髓组织工程有潜力彻底改变脊髓损伤治疗路径,现阶段实验研究主要以解决脊髓组织工程中的种子细胞与生物支架各自问题作为基础,进而深入探索二者适宜的复合模式,以此为临床应用提供更多基础证据。
Objective To explore the problems of seed cells and biological scaffolds in spinal cord tissue engineering,and review the recent experimental research.Methods Related literatures were searched in CNKI,Wangfang data,PubMed and Web of Science from establishment to March,2021,and the problems and progress of seed cells,biological scaffolds and their combination were reviewed.Results The problems of seed cells are carcinogenicity,immune rejection,ethics,low survival rate and differentiation rate after transplantation,and current researches focus on exploring new cell types,gene transfection,cell cotransplantation and pretreatment before transplantation.The problems of biological scaffold are that a single material selection cannot meet different needs,and the traditional technology cannot simulate the internal structure of spinal cord well.There were more researches focusing on new composite materials and new technology.The core problem of their combination is that the effects of different cell and scaffold combinations are different,and the current researches are mostly devoted to the continuous exploration of suitable composite mode,and try to introduce biological agents and other factors.Conclusion Spinal cord tissue engineering has the potential to completely change the therapeutic pathway of spinal cord injury.Current experimental researches mainly base on solving the problems of seed cells and biological scaffolds of spinal cord tissue engineering,and further explore the appropriate composite mode of seed cells and biological scaffolds,so as to provide more basic evidence for its clinical application.
作者
漆国栋
江琼
伍亚民
漆伟
QI Guo-dong;JIANG Qiong;WU Ya-min;QI Wei(Orthopedic Department,Chongqing Orthopedic Hospital of Traditional Chinese Medicine,Chongqing 400010,China;Chongqing Medical University,Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases,Chongqing 400016,China;Field Surgery Research Institute,Daping Hospital,PLA Military Medical University,State Key Laboratory of Trauma Burn and Complex Injury,Chongqing 400042,China)
出处
《中国康复理论与实践》
CSCD
北大核心
2021年第6期677-686,共10页
Chinese Journal of Rehabilitation Theory and Practice
基金
重庆市卫健委中医药科技项目(No.ZY201702134)
重庆市科卫联合中医药科研项目(No.2019ZY023495)。
关键词
脊髓组织工程
脊髓损伤
种子细胞
生物支架
实验研究
综述
spinal cord tissue engineering
spinal cord injury
seed cells
biological scaffolds
experiment research
review
作者简介
漆国栋(1993-),男,汉族,重庆市人,硕士,医师,主要研究方向:骨与神经组织损伤后修复方面的研究;通讯作者:漆伟(1966-),男,汉族,重庆市人,硕士,主任医师,主要研究方向:骨与神经组织损伤后修复方面的研究。E-mail:qiwei200@126.com。
