摘要
建立了QuEChERS前处理方法结合LC-MS/MS内标法测定水产品中17种喹诺酮类药物残留同时检测的方法。样品经含5%甲酸的乙腈提取,经EMR-Lipid dSPE净化,EMR polish脱水,浓缩定容后以电喷雾电离正离子(ESI+)、多反应检测模式(MRM)进行检测,内标法定量。该分析方法快速、准确,在大黄鱼、鲈鱼进行0.1μg/kg加标水平测试,其回收率为84.9%~111.5%,RSD为5.29%~22.66%。该方法适用于水产品中17种喹诺酮类药物的测定。
QuEChERS pretreatment method combined with LC-MS/MS was established for the simultaneous determination of 17 quinolones residues in aquatic products using internal standard method.The sample was extracted with acetonitrile containing 5%formic acid,purified by EMR-Lipid dSPE,dehydrated by EMR polish,concentrated and detected by electrospray ionization positive ion(ESI+)and multiple reaction detection mode(MRM),using internal standards.The method was rapid and accurate.Tests for recovery were made by addition of mix standards of 0.1μg·kg-1 in yellow croaker and perch.The recoveries were 84.9%~111.5%with RSD of 5.29%~22.66%.The method was suitable for the determination of 17 quinolones in aquatic products.
作者
朱品玲
钟茂生
翁才振
严飞
陈舒奕
陈珍珍
苏怡
ZHU Pin-ling;ZHONG Mao-sheng;WENG Cai-zhen;YAN Fei;CHEN Shu-yi;CHEN Zhen-zhen;SU Yi(Ningde Customs,Fujian Ningde 352100,China)
出处
《广州化工》
CAS
2021年第11期87-91,104,共6页
GuangZhou Chemical Industry
基金
福州海关科技计划项目(FK2017-ND001)。
关键词
液相色谱-串联质谱法
水产品
喹诺酮类药物残留
liquid chromatography-tandem mass spectrometry(LC-MS/MS)
aquatic products
quinolones residues
作者简介
第一作者:朱品玲(1979-),女,工程师,主要从事食品安全检测。
