摘要
为建立甲醇中磺胺类和喹诺酮类混合标准溶液的稳定性评定方法,使用液相色谱串联质谱法精确测定混合标准溶液中各组分含量随储存条件及储存时间的变化。结果表明,制备的混合标准溶液均匀性良好,各组分F值均小于95%置信水平下理论临界值;混合标准溶液中的磺胺嘧啶、磺胺喹噁啉、诺氟沙星和氟罗沙星在40℃储存条件下降解率大于10%;各组分浓度为10.0μg/mL的混合标准溶液在4℃避光储存,4个月内稳定性良好,超过4个月随储存时间延长,磺胺嘧啶、磺胺二甲基嘧啶、磺胺喹噁啉、诺氟沙星和氟罗沙星发生较明显的降解,其他组分含量呈现逐渐上升趋势。该混合标准溶液可用于食品和环境的残留检测及相关分析方法的确认与评价,有效期为4个月。
A method has been established for the stability evaluation of sulfanilamides and quinolones mixed standard solution in methanol.Each standard substance in the mixed standard solution was accurately determined by liquid chromatography tandem mass spectrometry(LC/MS/MS)in different storage conditions and different storage time.The results showed that the mixed standard solution demonstrated excellent homogeneity,with the F-value of each component falling below the theoretical critical value at the 95%confidence level.The solution rate of sulfadiazine,sulfaquinoxoline,norfloxacin and fleroxacin decreased by more than 10%at 40℃.Meanwhile,a mixed standard solution,with each component having a concentration of 10.0μg/mL,maintained satisfactory stability for up to four months when stored in darkness at 4℃.After more than 4 months,with the extension of storage time,the degradation of sulfadiazine,sulfamethazine,sulfaquinoxoline,norfloxacin and fleroxacin was more obvious,and the contents of other antibiotics showed a gradually increasing trend.This mixed standard solution has been found suitable for use in residue detection for food and environmental applications,assisting in the identification and evaluation of related analytical methods,with a validated effectiveness lasting for four months.
作者
黄鸾玉
吴祥庆
HUANG Luanyu;WU Xiangqing(Guangxi Academy of Fishery Sciences,Nanning 530021,China)
出处
《东北农业科学》
2023年第3期129-133,共5页
Journal of Northeast Agricultural Sciences
基金
广西壮族自治区科技重大专项(桂科AA20302019-1)
广西壮族自治区农业科技自筹经费项目(Z2023041、Z2023042)
关键词
磺胺类
喹诺酮类
混合标准溶液
标准物质
稳定性
Sulfonamides
Quinolones
Mixed standard solution
Reference material
Stability
作者简介
黄鸾玉(1983-),女,高级农艺师,硕士,从事水产品中痕量污染物检测技术研究。
