摘要
采用高速剪切技术对壳聚糖的机械降解过程进行了研究。考察了剪切时间、转速、温度、壳聚糖原溶液浓度、分子量和溶液pH等因素对壳聚糖降解效果的影响。以壳聚糖的动力黏度下降率来反映壳聚糖的降解程度,分别采用傅立叶变换红外光谱及凝胶渗透色谱对壳聚糖降解前后的结构和分子量分布进行了分析。研究结果表明,纯机械作用能够促进壳聚糖的降解,当壳聚糖原溶液浓度为3 g/L,分子量为100×10^(4),pH为4.6,温度为40℃,降解时间为40 min,转速为20000 r/min时,降解效果最明显,动力黏度下降率为20.27%。研究还发现黏度对壳聚糖的机械降解过程有一定影响,当壳聚糖原溶液黏度为4.0 mPa·s时,壳聚糖的黏度下降率最大。本文为深入探究壳聚糖降解机理提供了理论依据。
The mechanical degradation process of chitosan by high-speed shear technology was studied in this paper.The effects of shear time,rotational speed,temperature,initial concentration of chitosan solution,molecular weight of chitosan and pH of solution on the degradation of chitosan were investigated.The dynamic viscosity reduction rate of chitosan was used to reflect the degradation degree of chitosan.The structure and molecular weight distribution of chitosan before and after degradation were analyzed by fourier transform-infrared spectroscopy and gel permeation chromatography,respectively.The results showed that pure mechanical action could promote the degradation of chitosan.When the initial solution concentration was 3 g/L,molecular weight of chitosan was 100×10^(4),pH was 4.6,temperature was 40℃,shear time was 40 min,and rotational speed was 20000 r/min,the degradation effect was the most obvious,and the dynamic viscosity reduction rate was 20.27%.It was also found that the viscosity had a certain influence on the mechanical degradation process of chitosan.When the viscosity of chitosan solution was 4.0 mPa·s,the viscosity of chitosan decreased most.This paper provides a theoretical basis for further exploring the degradation mechanism of chitosan.
作者
曹艳
何向
刘德宇
黄永春
CAO Yan;HE Xiang;LIU Deyu;HUANG Yongchun(Department of Biological and Chemical Engineering,Guangxi University of Science and Technology,Guangxi Key Laboratory of Green Processing of Sugar Resources,Liuzhou 545006,China)
出处
《食品工业科技》
CAS
北大核心
2021年第11期38-43,共6页
Science and Technology of Food Industry
基金
国家自然科学基金(31660472)
广西高校中青年教师基础能力提升项目(2020KY08027)
广西科技基地和人才专项(桂科AD20238033)。
关键词
高速剪切
壳聚糖
降解
机械效应
动力黏度
high-speed shear
chitosan
degradation
mechanical effect
dynamic viscosity
作者简介
曹艳(1983−),女,博士,讲师,研究方向:生物资源化工与过程强化,E-mail:caoyan913@163.com;通信作者:黄永春(1974−),男,博士,教授,研究方向:生物资源化工,E-mail:huangyc@yeah.net。
