摘要
目的研究分析华佗豆的化学成分,为后续的开发和利用奠定基础。方法采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用法(UPLC-Q-Orbitrap HRMS),以正、负离子模式扫描采集数据,色谱条件为Thermo Gold C18色谱柱(2.1 mm×100 mm,1.9μm),以0.1%甲酸乙腈(A)-1%甲酸水(含10 mmol乙酸铵)(B)为流动相,流量为0.3 mL/min,进行梯度洗脱。通过分析质谱数据,参考相关文献以及谱库比对,对华佗豆的化学成分进行鉴定。结果共鉴定出华佗豆中的15个化合物,其中小檗碱、烟酰胺、咖啡因、柠檬酸、甘露醇、棉籽糖、乳果糖、羽扇豆醇、6,7-dehydro-11-hydroxy-12,13-epoxyterpendole A为首次从该植物中推测鉴定出来。结论建立的方法快速分析鉴别了华佗豆的化学成分,为华佗豆的质量控制提供科学依据。
Objective To study the chemical constituents of Calonyction muricatum(Linn)G.Don in order to lay the foundation for subsequent development and utilization.Methods Ultra-performance liquid chromatographyquadpole-orbitaltrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS)are used to collect data in positive and negative ion modes.Chemical constituents of Calonyction muricatum(Linn)G.Don.is performed on an Thermo Gold C18 column(2.1 mm×100 mm,1.9μm),mobile phase is consisted of 0.1%acetonitrile(A)-the 1%formic acid water(containing 10 mmol ammonium acetate)(B)with gradient elution.The flow rate is 0.3 mL/min.The chemical composition of Calonyction muricatum(Linn)G.Don is identified by relative molecular mass and combined with literature data and mass databases comparison.Results A total of 15 compounds in Calonyction muricatum(Linn)G.Don.are identified,including berberine,nicotinamide,caffeine,citric acid,mannitol,cottonseed sugar,lactulose,lupeol,6,7-dehydro-11-hydroxy-12,13-epoxyterpendole A are first identified from this plant.Conclusion The established method can analyze and identify the components rapidly,which provides scientific evidence for study on pharmacodynamics material of Calonyction muricatum(Linn)G.Don.
作者
林婧
梁洁
黄团心
黄光强
陈晓思
韦金玉
黄冬芳
LIN Jing;LIANG Jie;HUANG Tuan-xin;HUANG Guang-qiang;CHEN Xiao-si;WEI Jin-yu;HUANG Dong-fang(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Department of Pharmacy,Guangxi Agricultural Vocational College,Nanning 530007,China;Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Nanning 530200,China;Guangxi Institute for Food and Drug Control,Nanning 530021,China;Guangxi Superior Chinese Patent Medicine and National Medicine Development Engineering Technology Center,Nanning 530200,China)
出处
《现代中药研究与实践》
CAS
2021年第2期49-54,共6页
Research and Practice on Chinese Medicines
基金
广西一流学科建设开放课题(2019XK121)
广西研究生教育创新计划资助项目(YCSY2020012)
广西高等学校高水平创新团队及卓越学者计划项目(桂教师范〔2019〕52号)
广西高等学校千名中青年骨干教师培育计划(桂教人〔2019〕5号)
广西科技基地和人才专项(桂科AD20238058)。
作者简介
林婧(1993-),女,在读硕士研究生,研究方向:中药质量控制研究。E-mail:258576838@qq.com;通信作者:梁洁(1979-),女,博士,教授,硕士研究生导师,研究方向:中药药效物质基础及质量控制研究。E-mail:liangjie1101@126.com。
