摘要
[目的]以林木模式植物毛果杨为研究材料,旨在研究毛果杨MAP65基因家族成员的扩张与表达情况,为MAP65的功能研究提供参考。[方法]利用BLASTP基于Phytozome数据库鉴定毛果杨的MAP65基因家族成员,采用Prot-Param、Plant-mPLoc、LocTree3、Wolfpsort、MAGA7.0、GSDS、MEME、MCScanX、TBtools、BioEdit Sequence Alignment Editor、DnaSP5、plantCARE等工具分析其理化性质,预测其亚细胞定位,构建系统进化树,绘制基因结构图,分析保守结构域、基因复制事件、启动子元件,并基于基因芯片数据分析PtMAP65基因家族成员在不同组织中的表达量。[结果]利用BLASTP比对方法鉴定得到了9个MAP65基因。系统进化分析结果显示:植物MAP65基因家族分为5个亚组,且每个亚组都具有相似的基因结构和保守结构域。毛果杨基因组内共线性分析及同义突变频率(Ks)、非同义突变频率(Ka)分析结果表明:PtMAP65基因家族中有6对片段复制基因,且复制基因在进化过程中受到强纯化选择的作用。PtMAP65基因家族成员的表达数据分析显示:该基因家族成员在不同组织中有不同的表达模式,暗示其功能的分化。启动子的顺式作用元件分析表明:PtMAP65s的启动子中有较多的光响应元件和激素响应元件。[结论]片段复制是PtMAP65基因家族进化扩张的主要动力,推测基因家族成员之间存在功能分化。
[Objective] To study the expansion and expression of the MAP65 gene family members by using Populus trichocarpa, a model forest plant, as research material. [Method] Using BLASTP to identify the members of PtMAP65 gene family based on Phytozome database, and using tools such as Prot-Param, Plant-mPLoc, LocTree3,Wolfsort, MAGA7.0, GSDS, MEME, MCScanX, TBtools, BioEdit Sequence Alignment Editor, DnaSP5 and plantCARE to analyze the physical and chemical properties, predict subcellular locations, construct a phylogenetic tree,draw a gene structure diagram, analyze conserved domains, gene duplication events and promoter elements;and analyze the expression of PtMAP65 gene family members in different tissues based on gene chip data. [Result] Nine MAP65 genes were identified by BLASTP comparison. Phylogenetic analysis showed that the plant MAP65 gene family can be divided into 5 groups, and each group had a similar gene structure and conserved domain. The results of collinearity analysis and Ka and Ks analysis of the P. trichocarpa genome indicated that there were six pairs of segmental duplication genes in the PtMAP65 gene family, and the duplication genes were subject to strong purification selection in the evolutionary process. Analysis of the expression data showed that PtMAP65 gene family members had different expression patterns in different tissues, suggesting their functional divergence. Cis-acting element analysis of the promoter showed that PtMAP65 s promoter had more light response and hormone response elements than stress response, growth and development elements. [Conclusion] Segmental duplication is a main force driving the evolution and expansion of PtMAP65 gene family, and it is speculated that there has been functional divergence among the PtMAP65 gene family members.
作者
薛丽
刘晓霞
罗莹
吕中睿
张建国
饶国栋
XUE Li;LIU Xiao-xia;LUO Ying;LYU Zhong-rui;ZHANG Jian-guo;RAO Guo-dong(State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Co-Innovation Center of Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210000,Jiangsu,China;Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第2期92-101,共10页
Forest Research
基金
中央级公益性科研院所专项资金项目(CAFYBB2018QB001)。
作者简介
通讯作者:饶国栋,副研究员,硕士生导师.主要研究方向:林木遗传育种.Email:rgd@caf.ac.cn。
