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一种改进的观察植物细胞微管的免疫荧光染色方法 被引量：2

An Improved Immunofluorescence Microscopy for Observing Microtubule Cytoskeleton in Plant Cells
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摘要 植物微管体积小,而且始终处于动态变化之中,因此观察到清晰的微管形态有一定的难度。本文以拟南芥为实验材料,介绍了一种改进的植物细胞微管免疫荧光染色方法,用此方法可以观察到清晰、完整的植物微管形态,此法对其他植物细胞微管观察可能也有借鉴和参考价值。
作者 汪澈 张立军 李楠
机构地区 沈阳农业大学生物科学技术学院
出处 《植物生理学通讯》 CSCD 北大核心 2009年第7期699-701,共3页 Plant Physiology Communications
基金 国家自然科学基金(30800054) 辽宁省教育厅基金(2008656) 植物生理与生物化学国家重点实验室开放课题(PPB08002) 沈阳农业大学青年基金(2007)
关键词 微管 免疫荧光染色 拟南芥
分类号 Q94-33 [生物学—植物学]
作者简介 通讯作者（E-mail：wangwangche@163．com；Tel：024-88487163）。
  • 相关文献

参考文献2

  • 1张少斌,刘曦,张立军,汪澈,洪丽华.植物微管结合蛋白[J].植物生理学通讯,2009,45(3):291-298. 被引量：5
  • 2Yoshinobu Mineyuki. Plant microtubule studies: past and present[J] 2007,Journal of Plant Research(1):45～51

二级参考文献55

  • 1Anderhag P, Hepler PK, Lazzaro MD. (2000). Microtubules and microfilaments are both responsible for pollen tube elongation in the conifer Picea abies. Protoplasma, 214:141-157
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共引文献4

同被引文献15

  • 1简令成,孙龙华,林忠平.微管的冷稳定性与植物抗寒性关系的研究[J].Acta Botanica Sinica,1989,31(10):737-741. 被引量：9
  • 2Szechynska-Hebda M, Wedzony M, Dubas E, et al. Visualisation of microtubules and actin filaments in fixed BY-2 suspension cells using an optimised whole mount immunolabelling protocol[J]. Plant Cell Rep, 2006, 25(8) 758-766.
  • 3Abdrakhamanova A, Wang Q Y, Khokhlova L, et al. Is microtubule disassembly a trigger for cold acclimation?[J]. Plant Cell Physiol, 2003, 44(7): 676-686.
  • 4Komis G, Apostolakos P, Galatis B. Altered patterns of tubulin polymerization in dividing leaf cells of Chlorophyton comosum after a hyperosmotic treatment[J]. New Phytol, 2001, 149(2): 193-207.
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  • 8Qiao F. How cytoskeleton senses abiotic/biotic stimuli in plant cells? [D]. Germany : Karlsruhe Institute of Technology.. 2010 : 5-11.
  • 9Kobayashi Y, Kobayashi I, Funaki Y, et al. Dynamic reorganization of microfilaments and microtubules is necessary for the expression of non-host resistance in barley coleoptile cells[J]. Plant J, 1997, 11(3): 525-537.
  • 10Yoshida S, Handa Y, Suzuki T, et al. Microtubule-severing activity of Shigella is pivotal for intercellular spreading[J]. Science, 2006, 314:985-989.

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植物生理学通讯
2009年 第7期

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