摘要
采用反相高效液相色谱法,对钩藤不同部位异钩藤碱、钩藤碱、去氢钩藤碱、异去氢钩藤碱4种生物碱类成分进行含量测定。采用Gemini NX C18(250 mm×4.6 mm,5μm)色谱柱,以1%醋酸铵(A)-乙腈(B)为流动相进行梯度洗脱,流速1.0 mL·min^(-1);检测波长为245 nm;柱温25℃;进样量10μL。本方法简便可靠,专属性强,重现性好,适用于钩藤中生物碱的含量测定;钩藤不同部位生物碱类成分含量差异显著。
The contents of isorhynchophylline;rhynchophylline,corynoxeine and isocorynoxeine in different parts of Uncaria were determined by RP-HPLC.The method was performed on a Gemini NX C_(18) chromatographic column(250 mm×4.6 mm,5μm),with 1%ammonium acetate(A)-acetonitrile(B)as the mobile phase with gradient elution,the flow rate was 1.0 mL·min^(-1),the detect wavelength was 245 nm,the column temperature was 25℃,and the injection volume was 10μL.The method was simple,reliable,specific and reproducible.It was suitable for the determination of alkaloids in Uncaria.The contents of alkaloids in different parts of Uncariawere significantly different.
作者
刘小芬
蔡洪鲲
王琼
胡嘉璇
聂海澜
蒋范任
LIU Xiao-fen;CAI Hong-kun;WANG Qiong;HU Jia-xuan;NIE Hai-lan;JIANG Fan-ren(Yiyang Medical College,Hunan Yiyang 413000;Yiyang Institute for Food and Drug Control,Hunan Yiyang 413000,China)
出处
《广州化工》
CAS
2021年第8期153-155,共3页
GuangZhou Chemical Industry
基金
益阳市科技局项目(No.2017YZ18)
湖南省教育厅项目(No.18C1729)。
作者简介
第一作者:刘小芬(1979-)女,副教授,主管药师,主要从事药物质量分析及质量标准研究;通讯作者:蔡洪鲲(1979-),男,副主任药师,主要从事药物质量分析及质量标准研究;通讯作者:蒋范任(1981-),男,副主任药师,从事药品质量分析及质量标准研究。
