摘要
以离子液体的醇溶液为溶剂,采用超声辅助的方法提取栀子中的西红花苷,并用高效液相色谱仪测定提取液中西红花苷的含量,由此计算得出提取率。首先选择离子液体种类,再通过单因素实验确定料液比、乙醇体积分数、超声提取时间、离子液体浓度、超声提取温度的最佳参数。以单因素实验结论为基础设计响应面实验,利用Design-Expert 8.0软件对响应面模型进行分析,优化提取工艺的参数。结果表明,以离子液体([Bmim]BF_(4))的70%乙醇溶液为提取溶剂、离子液体浓度为0.82 mol/L、超声时间为41.76 min、料液比为1∶20.26(g/mL)为最佳提取条件,最高提取率可达到1.13%。与普通溶剂相比具有很大的优势。
The alcohol solution of ionic liquid was used as the solvent to extract the crocin from gardenia by ultrasound-assisted method,and the content of crocin in the extract was determined by HPLC,and the extraction rate was calculated.Firstly,the type of ionic liquid was selected,and then the optimal parameters of solid-liquid ratio,ethanol volume fraction,ultrasonic extraction time,ionic liquid concentration,and ultrasonic extraction temperature were determined by single factor experiment.The response surface experiment was designed on the basis of the single factor experimental conclusion,and the response surface model was analyzed by using the Design-Expert 8.0 software to optimize the extraction process parameters.The results showed that the best extraction conditions were 70% ethanol solution of ionic liquid([Bmim]BF_(4)),the ionic liquid concentration was 0.82 mol/L,the ultrasonic time was 41.76 min,and the ratio of solid to liquid was 1∶20.26(g/mL).The highest extraction rate was 1.13%.It has great advantages over common solvents.
作者
李晓严
郭蔚
刘鑫玉
LI Xiao-yan;GUO Wei;LIU Xin-yu(College of Science and Technology,Xinyang University,Xinyang 464000,China)
出处
《化学试剂》
CAS
北大核心
2021年第3期376-382,共7页
Chemical Reagents
基金
河南省高等学校重点科研项目(18B150025)
河南省高等学校青年骨干教师培养计划项目(2018GGJS199)。
关键词
离子液体
栀子
西红花苷
响应面法
高效液相色谱法
ionic liquid
gardenia
crocin
response surface method
high performance liquid chromatography
作者简介
通讯联系人:李晓严(1982-),女,河南周口人,硕士,副教授,主要从事绿色有机化学及新型有机材料研究,E-mail:xiaoyanli0901@126.com。
