摘要
【目的】探讨疏肝温胆汤对高脂膳食诱导的胰岛素抵抗肥胖大鼠糖脂代谢和炎症因子的影响。【方法】将40只SD大鼠随机分为正常组、模型组、中药组和他汀组,每组10只。除正常组,其他各组大鼠采用高脂膳食喂养法建立胰岛素抵抗肥胖模型。成功造模后,中药组和他汀组分别给予疏肝温胆汤和阿托伐他汀钙片混悬液灌胃4周,同期正常组和模型组给予等体积生理盐水灌胃。给药结束后,检测各组大鼠体质量、Lee’s指数、空腹血糖(FBG)、胰岛素(Fins)、血脂[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)],并计算稳态胰岛素抵抗指数(HOMA-IR)和胰岛素敏感性指数(QUICKI)、脂肪指数,以酶联免疫吸附分析(ELISA)检测脂肪组织炎症细胞因子[肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素17(IL-17)、白细胞介素1β(IL-1β)]含量。【结果】与正常组比较,模型组大鼠体质量、Lee’s指数、脂肪指数、HOMA-IR升高,QUICKI显著降低,血清FBG、Fins、TC、TG、LDL-C水平升高,HDL-C水平降低,脂肪组织TNF-α、IL-6、IL-17、IL-1β的含量升高(均P<0.05);与模型组比较,中药组大鼠体质量、Lee’s指数、脂肪指数、HOMA-IR降低,QUICKI显著升高,血清FBG、Fins、TC、TG、LDL-C水平降低,HDL-C水平升高,脂肪组织TNF-α、IL-6、IL-17、IL-1β的含量降低(均P<0.05),且除TC、LDL-C、HDL-C指标外,2个治疗组比较,差异均无统计学意义(P>0.05)。【结论】疏肝温胆汤可以改善胰岛素抵抗肥胖大鼠的糖脂代谢和抑制炎症反应状态。
Objective To explore the effects of Liver-Soothing and Gallbladder-Inducing Decoction on glucose and lipid metabolism and inflammatory factors in rats with insulin resistance obese induced by high-fat diet.Methods Forty SD rats were randomly divided into normal group,model group,Chinese medicine group and statin group,10 rats in each group.Apart from the normal group,the rats in the other groups were induced into insulin resistance obese model by feeding high fat diet.After successful modeling,the Chinese medicine group and statin group were given intragastric administration of Liver-Soothing and Gallbladder-Inducing Decoction and suspension of atorvastatin calcium tablets for 4 weeks,respectively,and the normal group and model group were given intragastric administration of the same volume of normal saline during the same period.After medication,the body mass,Lee’s index,fasting blood glucose(FBG),insulin(Fins),blood lipids[total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density Lipoprotein cholesterol(HDL-C)]were measured,and the steady-state insulin resistance index(HOMA-IR),insulin sensitivity index(QUICKI),and fat index were calculated.The contents of inflammatory cytokines[tumor necrosis factorα(TNF-α),interleukin 6(IL-6),interleukin 17(IL-17),interleukin 1β(IL-1β)]were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the normal group,the body mass,Lee’s index,fat index and HOMA-IR in the model group were increased,QUICKI was significantly decreased,serum levels of FBG,Fins,TC,TG and LDL-C were increased,HDL-C level was decreased,and the contents of TNF-α,IL-6,IL-17 and IL-1βin adipose tissue were increased(all P<0.05).Compared with the model group,the body mass,Lee’s index,fat index,HOMA-IR were decreased,QUICKI significantly was increased,serum FBG,Fins,TC,TG and LDL-C levels were decreased,HDL-C level was increased,and the contents of TNF-α,IL-6,IL-17,IL-1βin adipose tissue were decreased in Chinese medicine group(all P<0.05),there were no differences in other indexes except for TC,LDL-C,HDL-C between the two groups(P>0.05).Conclusion Liver-Soothing and Gallbladder-Inducing Decoction is effective for improving glucose and lipid metabolism and inhibiting the inflammatory state in rats with insulin-resistant obese.
作者
罗思聪
郭永宁
蔡海荣
蔡鑫桂
张为章
张烈元
陈伯钧
LUO Si-Cong;GUO Yong-Ning;CAI Hai-Rong;CAI Xin-Gui;ZHANG Wei-Zhang;ZHANG Lie-Yuan;CHEN Bo-Jun(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine/Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510006 Guangdong,China;The Second Clinical Medical School of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2021年第4期773-778,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省中医药局资助项目(编号:20211158)。
关键词
疏肝温胆汤
肥胖
胰岛素抵抗
糖脂代谢
炎症因子
大鼠
Liver-Soothing and Gallbladder-Inducing Decoction
obesity
insulin resistance
glucose and lipid metabolism
inflammatory factors
rats
作者简介
罗思聪(1986-),男,硕士,主治中医师,E-mail:3403171645@qq.com;通讯作者:蔡鑫桂(1989-),男,硕士,主治医师,E-mail:170775414@qq.com。
